高效液相色谱法测定益寿地仙颗粒中麦角甾苷

Determination of Acteoside in Yishoudixian Granule by High Performance Liquid Chromatography

目的:建立了用高效液相色谱法测定益寿地仙颗粒中麦角甾苷含量的方法。方法:实验采用C18柱,流动相:甲醇(A),乙腈(B),0.3%冰醋酸(C),梯度洗脱,在329nm检测波长处检测。结果:麦角甾苷的进样量为0.305～1.525μg时,进样量与色谱峰面积有良好的线性关系(r=0.9979);测得麦角甾苷平均回收率为113.03%,RSD为1.15%(n=5);方法的精密度好,相对标准偏差(RSD)为1.27%(n=5)。结论:方法快速、简便、准确,所测结果稳定、重现性好,可作为益寿地仙颗粒质量控制的一个检测方法。

Objective：A high performance liquid chromatographic method was established for the determination of acteoside in Yishoudixian Granule. Methods：The extracts were analysed on an RP-18column using a methanol-acetonitrile-0.1mol·L-1sodium acetate buffer aqueous HAc （42∶58,v/v） as the mobile phase with UV detection a 329nm. Results：A good linear response was observed within the range of 0.305～1.525μg of analytes （r=0.9979）. The average recovery of themethod was 113.03%,RSD=1.1% （n=5）,the relative standard deviation was1.27% （n=5）. Conclusion：This rapid,simple,accurate,and reproducible method is proposed for the quality control of Yishoudixian Granule.