大黄粗多糖传统水提-醇沉法最佳工艺研究

The Process of Rhubarb Thick Polysaccharide with Traditional Water Solution and Alcohol Sedimentation

目的:确定大黄多糖的最佳提取条件。方法:以大黄多糖的含量为指标,以提取温度、提取时间、提取次数、料液比为因素,利用正交试验法进行试验设计,对传统水提-醇沉法提取大黄多糖的工艺进行优化研究。结果:大黄粗多糖得率最高的提取条件是:提取时间3h,提取温度95℃,料液比1:30;大黄粗多糖纯度最好的条件是:提取时间1h,提取温度95℃,料液比1:10,乙醇沉淀大黄多糖的最佳浓度是80%。结论:本方法实验结果可作为提取大黄多糖最佳工艺的依据。

[Objective] To determine the optimum extracting conditions of rhubarb polysaccharide. [Methods] The process of extracting rhubarb polysaccharide with the traditional water solution and alcohol sedimentation method was studied by orthogonal test, taking the content of rhubarb polysaccharide as standards, which included the factors such as extraction temperature, extraction time, extraction times and ratio of material to liquid. [Results] The optimum extraction condition of rhubarb thick polysaccharide has been established as follows： extraction time 3 hours, extraction temperature 95 ~C, and ratio of material to liquid 1 ： 30. The optimum purity condition of rhubarb thick polysaccharide has been established as follows： extraction time 1 hour, extraction temperature 95 ℃, and ratio of material to liquid 1 ： 10. The best density of ethanol depositing rhubarb polysaccharide is 80%. [Conclusion] The experimental result of this method could be used as the basis of extracting rhubarb polysaccharide process.