茶橘片对酒精性肝损伤的保护作用研究

Protective Effects of Tea-Orange Tablets on Alcoholic Hepatic Injury

本论文通过建立小鼠急性酒精性肝损伤、大鼠慢性酒精性肝损伤动物模型,考察了沙田柚皮和茶叶提取物的配伍制备茶橘片对急慢性酒精性肝损伤的保护作用。50%乙醇(0.01mL/g)灌胃小鼠,每天给予两次,共10d,可成功建立小鼠急性酒精性肝损伤模型。结果表明:与模型组比较,茶橘片(100mg/kg、300mg/kg、900mg/kg)剂量能显著降低血清TG含量,减少肝组织MDA的生成,提高GSH-ST的活性,呈剂量依赖关系(P<0.001)。采用酒精灌胃(50%乙醇,4g/kg),喂养高脂饲料,腹腔注射微量CCl4(25%CCl4-花生油溶液,0.1mL/kg)的方法八周可成功建立慢性酒精性肝损伤大鼠模型。与模型组比较,茶橘片(70mg/kg、210mg/kg、630mg/kg)可显著降低血清ALT水平,增加血清ALB含量,减少肝组织MDA及CHO的生成,增加SOD的活性,呈剂量依赖关系(P<0.001)。茶橘片由于富含天然黄酮类活性物质能够减轻酒精对脂肪代谢的影响,对急慢性酒精性肝损伤起保护作用。

Tea-Orange Tablets were made from extracts of Satian pomelo and tea. Acute and chronic alcoholic hepatic injury animal models were setup to test the protective effects of tea-orange tablets from pomelo peel and tea extract. Acute alcoholic hepatic injury mice models were setup by oral administration of 50% alcohol twice a day for 10 days. Compared to control group, tea-orange tablets （100 mg/kg, 300 mg/kg and 900 mg/kg） could reduce serum TG and MDA contents, and improve activity of GSH-Px, showing dose dependence （p〈0.001）. Chronic alcoholic hepatic injury rat models was setup by oral administration of 50% alcohol （4 g/kg, p.o.） and 25% CC14 （0.1 mL/kg, i.p.） for 8 weeks. Oral administration of tea-orange tablets （70, 210 and 630mg/kg） could significantly reduce contents of ALT, MDA and CHO, and improve contentsof ALB and activity of SOD （p〈0.001）. Tea-orange tablets were in rich of flavones material and had protective effects to acute and chronic alcoholic hepatic injury.