高效液相色谱法测定地黄丸类中成药中丹皮酚含量

High performance liquid chromatographic determination of paeonol contents in Dihuang pills

用高效液相色谱法对不同品牌和类别地黄丸中的丹皮酚进行了测定。考察了甲醇、乙醇、氯仿、四氯化碳4种溶剂提取丹皮酚的效果,综合考虑提取剂毒性、提取率和对分离的影响,选择甲醇作提取剂。对色谱方法的稳定性、准确度、精密度和线性进行了考察。对不同品牌和类别地黄丸中的丹皮酚含量进行了比较分析,各品牌有较大差别。产生差别的主要原因是药材产地和组方不同。本方法可作为地黄丸生产中的质量控制方法。

The paeonol contents in Dihuang pills of different brands and categories were determined by high performance liquid chromatography （HPLC）. The effects of four different solutions （methanol, ethanol, chloroform, and tetrachloromethane） for the extraction of paeonol were investigated. With the consideration of toxicity, extraction ratio, and the influence on separation, methanol was selected as extraetant. The stability, accuracy, precision, and linearity of the HPLC method were studied also. The paeonol con- tents in different brands and categories of Dihuang pills were compared, and the results showed that they were quite different. The main causes are that the raw materials are produced in different places, and the compositions of different brands are different. The method can be used for quality control in the production of Dihuang pills.