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川芎嗪对白血病K562细胞增殖和凋亡的影响 被引量：3

The effect of Tetramethylpyrazine on telomerase activity and apoptosis in leukemia K562 cells
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摘要 目的:探讨川芎嗪对K562细胞端粒酶活性及凋亡的影响,进一步研究川芎嗪抗白血病作用的分子机制。方法:用光学显微镜及透射电镜观察经川芎嗪作用后K562细胞具有的凋亡形态学改变,采用流式细胞仪检测Fas蛋白的表达;采用多聚酶链式反应-酶联免疫测定法(PCR-ELISA)检测白血病细胞端粒酶活性。结果:川芎嗪能抑制K562细胞端粒酶活性,随药物浓度增加和利用时间的延长,其抑制作用增强,并能诱导K562细胞凋亡,使Fas蛋白水平上调。结论:川芎嗪能显著抑制K562细胞端粒酶活性,其作用可能与诱导细胞凋亡有关。在此过程中,Fas也可能参与了细胞凋亡的调节。 Objective：To investigate the effects of Tetramethylpyrazine（TMP）on telomerase activity and apoptosis in K562 cells lines and study the melocular mechanism of it＇s antileukemie. Methods： Cell apoptosis was observed by light microscopy and transmission electron microscopy. The expression of Fas was measured by flow cytometry. Polymerase chain reaction enzyme linked immunoassay（PCR-ELISA）was used to assay telomerase activity of K562 cells. Results： Tetramethylpyrazine could inhibit telomerase activity of K562 cells in a time-and concentrationdependentmanner. Meanwhile,it could induce apoptosis obviously and up-regulate the levels of Fas in K562 cells. Conclusions：Tetramethylpyrazine could inhibit telomerase activity of K562 cells, and the mechanism of effect was maybe relate to inducing apoptosis and the expression of Fas protein.
作者 吴涛 瞿桂芳
机构地区 武钢总医院血液科
出处 《中国冶金工业医学杂志》 2010年第1期5-7,共3页 Chinese Medical Journal of Metallurgical industry
关键词 川芎嗪 白血病K562细胞 端粒酶 细胞凋亡 Tetramethylpyrazine K562 leukemic eells Telomerase Apoptosis
分类号 R285.5 [医药卫生—中药学]
  • 相关文献

参考文献12

  • 1Norrback KF, Roos G. Telomere and telomerase in normal and malignanthaematopioetic cells. Eur J Cancer, 1997, 33 (5): 774- 780.
  • 2李乃农,陈元仲,吕联煌.白血病细胞端粒酶活性的研究[J].白血病．淋巴瘤,2002,11(5):279-281. 被引量：5
  • 3Seol JG, Kim ES, Park WH, et al. Telomerase activity in acute myelogenousleukemia: clinical and biological implications. Br J Haematol,1998,100(1) : 156 165.
  • 4Bdlamy WT, Richter L, Frutiger Y, et al. Expression of vascular endothelial growth factor and its receptors in hematopoietic malig nancies[J]. Cancer Res, 1999,59(3): 728.
  • 5陈光浩,况立革.血清血管内皮生长因子与肿瘤[J].解剖科学进展,2002,8(4):364-366. 被引量：7
  • 6Wang X, Chen XX. Study survey and prospect of effect of ligusrazine on caner cell[J]. China J Chin Mater Med,2003,28(4) : 295.
  • 7Chinni SR, Alhasam SA, Multani AS, et al. Pleotropic effects of genistein on MCF-7 breast cancer cells[J]. Int J Mol Med,2003,12 (1) :29-34.
  • 8Nakayama Y, Sakamato H, Satoh K, et al. Tamoxifen and gonadal steroids inhibit colon can cer growth in association with inhibition of thymidylate synthase, survivin and and telomerase expression through estrogen receptor beta mediated system. Cancer Lett, 2000,161(1) : 63-71.
  • 9肖扬,张洹.人端粒酶RNA反义寡核苷酸对顺铂诱导K562细胞凋亡的影响[J].第一军医大学学报,2003,23(2):144-147. 被引量：6
  • 10Iida A,Yamaguchi A, Hirose k. Telomerase activity in clolorectal cancer ant its relationship to bel-2 expression. J Surg Oncol,2000, 73(4) : 219-223.

二级参考文献49

  • 1[1]Shay JW, Bacchetti S. A survey of telomerase activity in human cancer[J]. Eur J Cancer, 1997, 33(5): 787-91.
  • 2[2]Kyo S, Takakura M, Inoune M. Telomerase activity in cancer as a diagnostic and therapeutic target [J]. Histol Histopathol, 2000, 15(3): 813-24.
  • 3[3]Feng J, Funk WD, Wang SS, et al. The RNA component of human telomerase[J]. Science, 1995, 269(5228): 1236-41.
  • 4[4]Villa R, Folin M, Lualdi S, et al. Inhibition oftelomerase activity by a cell-penetrating peptide nucleic constract in human melanoma cells[J]. FEBS Lett, 2000, 473(2): 241-8.
  • 5[5]Naka K, Yokozaki H, Yasai W, et al. Effect ofantisense human telomerase RNA transfection on the growth of human gastric cancer cell lines[J]. Biochem Biophys Res Commun, 1999, 255(3): 753-8.
  • 6[6]Zhang JX, Zhang ZK, Sheng HP, et al. Telomerase activity is not related to apoptosis in leukemic cell lines [J]. Life Sci, 2000, 66(18): 1713-23.
  • 7[7]Yang P, Becker D. Telomerase activity and expression of apoptosis and anti-apoptosis regulators in the progression pathway of human melanoma[J]. Int J Oncol, 2000, 17(5): 913-9.
  • 8[8]Kushner DM, Paranjape JM, Bandyopadhyay B, et al. 2-5A antisense directed against telomerase RNA produces apoptosis in ovarian cancer cells[J]. Gynecol Oncol, 2000, 76(2): 183-92.
  • 9[9]Kondo Y, Kondo S, Tanaka Y, et al. Inhibition oftelomerase increases the susceptibility of human malignant glioblastoma cells to cisplatin-induced apoptosis[J]. Oncogene, 1998, 16(17): 2243-8.
  • 10[10]Kondo Y, Komata T, Kondo S. Combination therapy of 2-5A antisense against telomerase RNA and cisplatin for malignant gliomas[J]. Int J Oncol, 2001, 18(6): 1287-92.

共引文献15

同被引文献32

  • 1MIRI-MOGHADDAM E, DEEZAGI A, SOHEILI ZS, et al. Apoptosis and reduced cell proliferation of HL-60 cell line caused by human te- lomerase reverse transcriptase inhibition by siRNA [ J]. Acta Haema- tol,2010,124(2) :72-78.
  • 2WHITE LK, WRIGHT WE, SHAY JW. Telomerase inhibitors [ J ]. Trends Biotechnology ,2001,19 ( 3 ) : 114-120.
  • 3FAN Y, LIU Z, FANG X, et al. Differential expression of full-length telomerase reverse transcriptase mRNA and telomerase activity be- tween normal and malignant renal tissues [ J 1. Clinical Cancer Re- sarch ,2005,11 ( 12 ) :4 331-4 337.
  • 4FUJIMOTO K, TAKAHASHI M. Telomerase activity in human leuke- mic cell lines is inhibited by antisense pentadecadeoxynucleotides targeted against c-myc mRNA[ J]. Biochemical and Biophysical Re- sarch Communications, 1997,241 ( 3 ) :775-781.
  • 5MEYERSON M, COUNTER CM, EATON EN, et al. hEST2, the pu- tative human telomerase catalytic subunit gene, is up-regulated in tumor cells and during immortalization[ J ]. Cell, 1997,90 (4) :785- 795.
  • 6TAKAKURA M, KYO S, KANAYA T, et al. Cloning of human telom- erase catalytic subunit (hTERT) gene promoter and identification of proximal core promoter sequences essential for transcriptional activa- tion in immortalized and cancer cells [ J ]. Cancer Research, 1999,59 (3) :551-557.
  • 7PAPANIKOLAOU V, ATHANASSIOU E, DUBOS S, et al. hTERT regulation by NF-kappaB and c-mye in irradiated HER2-positive breast cancer ceils [ J ]. International journal of Radiation Biology, 2011,87(6) :609-621.
  • 8SAGAWA Y, NISHI H, ISAKA K, et al. The correlation of TERT ex- pression with e-mye expression in cervical cancer [ J ]. Cancer Let- ters,2001,168( 1 ) :45-50.
  • 9LONG J, PARKIN B, OUILLETTE P, et al. Multiple distinct molecular mechanisms influence sensitivity and resistance to MDM2 inhibitors in adult acute myelogenous leukemla[J]. Blood, 2010, 116(1 ):71-80.
  • 10WIDMANN C, GERWINS P, JOHNSON N L, et al. MEK kinase 1, a substrate for DEVDdirected caspases, is involved in genotoxininduced apoposis[J]. Mol Cell Biol, 1998, 18(4):2416-2429.

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二级引证文献7

中国冶金工业医学杂志
2010年 第1期

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