老年人肺炎克雷伯菌分离株氨基糖苷类修饰酶基因研究

RESISTANCE AND GENOTYPE OF AMINOGLYCOSIDE MODIFYING ENZYMES IN CLINICAL ISOLATES OF KLEBSIELLA PNEUMONIAE FROM HOSPITALIZED ELDERLY PATIENTS

目的了解临床分离的肺炎克雷伯菌耐药性及氨基糖苷类修饰酶基因的存在状况。方法测定临床分离的40株肺炎克雷伯菌对20种抗菌药物的敏感性,采用PCR技术检测氨基糖苷类修饰酶基因。结果 40株肺炎克雷伯菌呈现多重耐药,氨基糖苷类修饰酶基因aac(3)-Ⅰ、aac(3)-Ⅱ、aac(6′)-Ⅰ b和aac(6′)-Ⅱ的阳性率分别为12.5%、75.0%、7.5%和10.0%。携带2种或2种以上基因的菌株有10株(25.0%)。结论临床分离的肺炎克雷伯菌多重耐药严重,氨基糖苷类修饰酶基因携带较高。

Objective To investigate the resistance and genotype of aminoglycoside modifying enzymes（AMEs） in clinical isolates of Klebsiella pneumoniae. Methods Microdilute tests were performed to detect susceptibility of 20 kinds of antimicrobial agents in 40 isolates of Klebsiella pneumoniae. Genotypes of AMEs were detected by PCR. Results The 40 isolates showed multiple drug resistanee. The positive rates of aac（3）-Ⅰ , aac（3）-Ⅱ ,aac（6＇）-Ⅰ b,and aac （6＇）-Ⅱ genes related to AMEs were 12.5% ,75.0% ,7.5% and 10.0% ,respectively. There were 10 strains （25.0%） carrying two or more genes. Conclusion The Clinical isolates of Klebsiella pneumoniae exhibited strong multidrug resistance, and there were high positive percentages of genotypes of the isolates from hospitalized elderly patients in AMZs.