维生素E对光照疗法诱导的新生儿氧化应激反应预防作用研究

THE PREVENTIVE EFFECT OF VITAMIN E ON OXIDATIVE STRESS INDUCED BY PHOTOTHERAPY IN NEONATES

目的探讨维生素E(VitE)对光照疗法(光疗)诱导的新生儿氧化应激反应的预防作用。方法将60例非溶血性高胆红素血症新生儿随机分为2组,光疗+VitE组30例,入院后立即光疗24 h,光疗前口服VitE 50mg;光疗组30例,入院后立即光疗24 h,光疗前不用VitE。用比色法测定血清丙二醛(malondialdehyde,MDA)浓度、血清超氧化物歧化酶(superoxide dismutase,SOD)、过氧化氢酶(catalase,CAT)、谷胱甘肽过氧化物酶(glutathione peroxidase,GSH-Px)活性。结果光疗+VitE组光疗24 h后血清MDA浓度、SOD、CAT、GSH Px活性与光疗前差异无统计学意义(P>0.05);光疗组光疗24 h后血清MDA浓度较光疗前显著升高(18.59±3.11/16.13±3.12)mmol/L(P<0.01);血清SOD、CAT、GSH Px活性较光疗前下降,分别为(63.92±12.40/75.80±13.78)kU/L,(1.60±0.44/1.87±0.51)kU/L,(74.33±31.48/90.52±29.88)酶活力单位(P<0.01)。结论 VitE对光照疗法诱导的新生儿氧化应激反应具有预防作用。

Objective To explore the role of vitamin E in preventing oxidative stress induced by phototherapy in neonates with hyperbilirubinemia. Methods Sixty neonates with hyperbilirubinemia were divided into 2 groups randomly. The phototherapy ＋ VitE group （30 cases）：24 hours of phototheraphy were given after admission and vitamin E 50mg were taken orally before phototherapy. The phototherapy group（30 cases）：24 hours of phototherapy were given after admission and were not given vitamin E before phototherapy, malondialdehyde （MDA）, superoxide dismutase （SOD）, catalase （CAT） and glutathione peroxidase （GSH-Px） activities in serum were measured by chromatometry. Results In the phototherapy ＋vitE group, the serum level of MDA and the activities of SOD,CAT and GSH-Px were not different between before and after 24 hours of phototherapy （ P 〈0.05）. In phototherapy group the serum level of MDA after 24 hours of continuous phototherapy was significantly higher than that before phototherapy （18.59±3.11 vs 16.13±3.12）mmol/L（ P 〈0.01）,the serum activities of SOD, CAT and GSH-Px were significantly lower than those before phototherapy[（63.92±12.40 vs 75.80±13.78）kU/L, （1.60±0.44 vs 1.87±01 51）kU/L, （74.33±31.48 vs 90.52±29.88） unit of activity] （ P 〈0.01）. Conclusion Vitamin E have preventing role to oxidative stress induced by phototherapy in neonates.