慢性HBV感染患者外周血CD4^+CD25^+调节性T细胞表达及其临床意义

Expression levels of CD4^+CD25^+ regulatory T cells in peripheral blood mononuclear cells in patients with chronic hepatitis B viral infection

目的探讨CD4+CD25+调节性T细胞与慢性HBV感染后不同临床转归和临床特点的相关性。方法在26例慢性乙型肝炎(CHB)患者、15例无症状HBsAg携带者(ASC)和11例肝炎肝硬化(LC)患者和16例正常对照者,分离外周血单个核细胞(PBMC),采用流式细胞仪检测CD4+CD25+调节性T细胞的表达水平。结果CHB组和ASC组的CD4+CD25+调节性T细胞占CD4+T细胞的百分率分别为4.40±2.76%和4.43±2.10%,均高于正常对照组(2.70±0.97%),差异显著(P<0.01);CD4+CD25+调节性T细胞的表达水平与HBVDNA水平无相关性(r=0.018,P>0.05);在HBeAg阳性与阴性组患者CD4+CD25+调节性T细胞的表达也无明显的差异(P>0.05)。结论慢性HBV感染者外周血CD4+CD25+调节性T细胞水平升高,可能与HBV感染的慢性化有关。

Objective To explore the relationship between CD4^＋CD25^＋ regulatory T cells and different clinical outcomes after HBV infection. Methods Fresh isolated peripheral blood mononuclear cells（PBMCs） in 26 patients with chronic hepatitis B（CHB）, 15 with asymptomatic HBsAg carrier（ASC）, 11 with liver cirrhosis（LC）and in 16 healthy donors were analyzed for the proportion of CD4^＋CD25^＋ regulatory T cells by using flow cytometry. Results The proportions of CD4^＋CD25^＋ regulatory T cells to CD4^＋ T cells in patients with CHB （4.40±2.76%） and ASC （4.43±2.10%） were higher than those in healthy donors （2.70±0.97%,P〈0.01）;the percentage of CD4^＋CD25^＋ regulatory T cells was not correlated with serum HBV DNA level （r=0.018,P〉0.05） or serum HBeAg positive or negative states （P〉0.05）. Conclusion The level of CD4^＋CD25^＋ regulatory T cells in CHB patients was increased. It may be related to the chronicity of HBV infection.