慢性乙型肝炎患者HBV基因型和亚型分布调查

Prevalence of hepatitis B virus genotypes and subtypes in patients with chronic hepatitis B

目的研究慢性乙型肝炎患者HBV基因型和亚型流行情况。方法应用HBV基因型和亚型特异性引物PCR法对北京、长春、大连、西安、石家庄、郑州和合肥7个城市660份HBVDNA阳性慢性乙型肝炎患者血清进行基因型和亚型分析。结果在660份HBVDNA阳性血清中,B基因型、C基因型和B/C混合感染分别为16.67%(110/660)、74.54%(492/660)和8.79%(58/660);在C基因型中,C1亚型6例(1.22%)、C2亚型473例(96.14%)、C1/C2混合基因亚型13例(2.64%);B基因型均为Ba亚型,B基因型和C基因型混合感染者均为Ba与C2亚型混合感染,未发现其他基因型和基因亚型;不同基因型感染患者HBeAg阳性率差异无统计学意义(P=0.153);B基因型和C基因型患者之间血清HBVDNA水平差异无统计学意义(6.37±1.62lgcopies/ml对6.29±1.76lgcopies/ml),但均高于B和C基因型混合感染患者(5.25±1.65lgcopies/ml)。结论这7个城市慢性乙型肝炎患者以B基因型和C基因型感染为主,有部分B/C基因型混合感染。HBV亚型以Ba和C2亚型占优势。

Objective To study hepatitis B virus （HBV） genotypes and subtypes distribution in chronic hepatitis B patients. Methods Genotype/subtype-specific primer PCR was used to detect HBV genotypes and subtypes in 660 chronic hepatitis B patients from Beijing（n=474）,Shijiazhuang（n=40）,Changchun（n=55）,Dalian （n=20）,Zhengzhou（n=33）, Xi＇an（n=26） and Hefei（n=12）. Results. Among 660 patients with chronic hepatitis B （all with serum HBV DNA positivet,the prevalences of genotype B,C,and B/C subtype were 16.67%（110/660）, 74.54% （492/660）, and 8.79% （58/660）, respectively; In genotype C-infected patients, 6 （ 1.22% ） were subtype C 1, 473 （96.14%） were subtype C2,and 13 （2.64%） were C1/C2 coinfection;All genotype B were subtype Ba;There was no significant difference of HBeAg positivities or serum HBV DNA levels between subtype B and subiype C infected patients. Conclusion The common HBV genotypes in these seven cities are genotype B and C,and genotype C prevail,while subtypes Ba and C2 are predominant.