用于检测毫微微克分子浓度抗原的基于光钳原理的免疫传感器(英文)

Optical Tweezers-based Immunosensor for Detection of Femtomolar Concentrations of Antigens

超低浓度分析试样的测定已日显重要。特别在生物样品的临床检测方面的需求更显迫切。作者所进行的一系列研究中应用了对单个抗原—抗体键敏感的光学捕获技术,对蛋白质抗原的检测下限可达毫微微克分子浓度。应用激光光束的光箝装置被用于捕获及远程调控介电粒子,如细胞及其它生物体,由此而引起的动量改变可导致折射率的变化而被检测。光箝原理与免疫技术的结合可用于免疫检测。免疫技术中的竞争耦合置换被用于检测溶液中的微量抗原。BSA抗原被用作检测实例。实验结果表明:检测下限可达1.45×10^(15)mol/L,亦即66个BSA分子。

Detection of ullralow concentrations of analytes arc becoming increasingly important. Methods that do not require amplification techniques to detect infectious organisms, virus, and nucleic acid targets could find wide applications in the clinical environment. Optical tweezers were used for sensor construction in author's lab. Optical tweezers arc focused laser beams used to trap and remotely manipulate dielectric particles, including cells and other biological objects. The change in momentum of the light transmitted by the dielectric object results in a force that traps objects having an index of refraction greater than that of the surrounding medium at the local maximum of the intensity of the electromagnetic field, i.e. the focus of the laser beam.