食管癌中CTGF,Cyr61表达与相关因素分析

Expression of Cyr61 and CTGF in human esophageal cancer tissues and their correlative factors

目的探讨人食管癌组织中结缔组织生长因子(CTGF),富含酪氨酸61(Cyr61)表达及其与相关因素的关系。方法采用实时荧光定量PCR和免疫组化染色法对186例食管癌患者的食管癌组织和癌旁正常食管组织中CTGF,Cyr61表达水平进行检测,并进行Logistic回归分析。结果与正常食管组织比较,食管癌组织中CTGF,Cyr61 mRNA(5.29±5.12,7.13±4.26)均表达上调(P=0.000),蛋白均高表达(20.83±8.20,16.79±6.81)(P=0.000);多因素Logistic回归分析显示:吸烟,浸润深度是CTGF mRNA表达的危险因素(P<0.01);吸烟、淋巴结转移、家族史、肿瘤大小、病理分级是CTGF蛋白表达的危险因素(P<0.05);淋巴结转移、肿瘤大小、浸润深度是Cyr61 mRNA表达的危险因素(P<0.05);淋巴结转移、病理分级、浸润深度是Cyr61蛋白表达的危险因素(P=0.001)。结论CTGF、Cyr61在食管癌发展过程中起重要作用,其可能成为基因治疗的重要靶点。

Objective To investigate connective tissue growth factor（CTGF）,cystein-rich 61（Cyr61） expression levels and their correlative factors in human esophagus carcinomas.Methods We quantified CTGF,Cyr61 expression levels in 186 esophageal cancer tissues and normal esophagus tissues around the tumor with real-time PCR assay and immunohistochemistry staining and analyzed correlative factors by logistic regression.Results Compared with normal esophagus tissues,up-regulations of CTGF,Cyr61 mRNA（5.29±5.12,7.13±4.26） were found in esophageal cancer tissues（P=0.000）.The CTGF,Cyr61 protein expression levels of esophageal cancer tissues （20.83±8.20,16.79±6.81） were significantly higher than that of in the normal esophagus tissues （P=0.000）.Multiple factor logistic regression showed that in esophageal cancer,smoking and infiltrating depth were the risk factors of CTGF mRNA expression （P0.01）. Smoking,metastasis,family history,tumor size and tumor stage were the risk factors of CTGF protein expression （P0.05）. Metastasis,tumor size and infiltrating depth were the risk factors of Cyr61 mRNA expression（P0.05）. Metastasis,tumor stage and infiltrating depth were the risk factors of Cyr61 protein expression（P=0.001）.Conclusion CTGF,Cyr61 may play a role in the progression of esophageal cancer,and may be an important target for gene therapy.