产丁醇芽孢杆菌的分离、筛选与鉴定

Screening and Identification of a Bacillus Strain Producing Butanol

通过富集培养和分离纯化等过程,从种植怀地黄的土壤中分离得到一株产丁醇兼性厌氧细菌菌株C2。以7%的玉米醪液为原料总溶剂(丙酮、丁醇、乙醇,ABE)产量可达17.17g/L,其中丁醇11.2g/L,占65.2%;发酵玉米秸秆糖化液(总糖浓度为25g/L)产总溶剂量为3.64g/L,其中丁醇2.63g/L,占72.3%。形态学、生理生化及系统发育研究表明该菌株为革兰氏阳性芽孢杆菌(Bacillus),与B.vallismortis、B.atrophaeus和B.mojavensis亲缘关系最近。

A facultative anaerobic butanol producing bacteria named C2 was isolated from soil planted with rehmanniae. C2 could produce 17.17 g/L total solvent （acetone, butanol and ethanol, ABE） in a 7% corn mash and the butanol yield reached 11.2 g/L amounting to 65.2% of the total solvent. Using com straw hydrolysate as raw material （total sugar 25 g/L）, 3.64 g/L total solvent was produced, among which 72.3% was butanol, reached to 2.63 g/L. According to the analysis of morphology, physiological and biochemcal characteristics and 16S rDNA sequence, this bacteria strain was identified as Bacillus sp., and was most related to B. vallismortis, B. Atrophaeus and B. mojavensis.