小干扰RNA逆转人乳腺癌细胞MCF-7多药耐药的体外研究

Study on the reversal of multidrug resistance of human breast cancer cell line MCF-7 by siRNA in vitro

目的研究以多药耐药相关蛋白(MRP)为靶标的小干扰RNA(siRNA)抑制相应蛋白表达,对人乳腺癌细胞MCF-7药物敏感性的影响。方法以MRP基因为靶标的siRNA转入MCF-7细胞,检测对MRP mRNA和蛋白表达水平的影响及其对抗肿瘤药物IC50的影响。结果经siRNA干扰后,MRP基因的mR-NA和蛋白的表达均有明显降低,肿瘤细胞对相关化疗药物的敏感性明显增加。结论以MRP基因为靶标的siRNA一定程度上逆转了肿瘤细胞的多药耐药性。

Objective To study on the regulation of muiltidrug resistance in MCF- 7 cells after transfecting （small interfering RNA ） siRNA targeted on MRP. Methods Small interfering RNAs （ siRNA ） targeted on MRP were transfected into MCF-7 cells, then the expression level of the MRP mRNA and protein was determined, the cellular sensitivity to cheomotherapeutics was detected. Results After siRNA interfering, the level of MRP mRNA and protein was decreased , meanwhile, the tumor cells＇ sensitivity to cheomotherapeutics obviously increased. Conclusion To some extent, the muhidrug resistance of MCF -7 cell line was reversed.