HPLC法测定茴三硫片中茴三硫的含量

Determination of anethole trithionein in anethole trithione tablets by HPLC

目的建立测定茴三硫片中茴三硫含量的高效液相色谱法。方法采用高效液相色谱法，以C18为色谱柱，甲醇-水（体积比80：20）为流动相，流速为1．0ml·min^-1，检测波长为348nm。结果茴三硫在10．5—50．24mg·L^-1的浓度范围内具有良好的线性关系，r=0．9998。茴三硫加样回收率分别为99．2％（n=5，RSD=0．49％）。结论本法操作简便，灵敏度高，重复性好，测定结果准确，可用于茴三硫的质量控制。

Aim To establish an HPLC method for determination of anethole trithione in anethole trithione tablets. Method The chromatography column is C18 （250 mm× 4.6 mm,5 μm） , with a mobile phase consisting of methanol-water（ 80：20 ）. The flow-rate is 1.0 ml ·min^- 1. The UV detection wavelength is set at 348 nm. Results The linear range of anethole trithione is 10.5 - 50, 24 mg·L^- 1, r = 0. 999 8 （ n = 5 ）. The average recovery was 99.8% , with an RSD of 0.51%. Conclusion The method was proved to be simple, rapid and accurate for the quality control of an ethole trithione tablets.