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拟南芥AtCpNifS克隆及其原核表达 被引量：2

Cloning and Prokaryotic Expression of AtCpNifS from Arabidopsis thaliana
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摘要 从哥伦比亚生态型拟南芥(Arabidopsis thaliana ecotype Columbia)中克隆了AtCpNifS的完整CDS序列。通过测序和序列比对分析,确认了克隆序列的完整性和正确性。在此基础上,构建了该基因的原核表达载体(BL21-pET28-AtCpNifS),并从该质粒中成功分离了表达蛋白(AtCpNifS)。 The whole coding domain sequence （CDS） of AtCpNifS was isolated from Arabidopsis thaliana ecotype Columbia. The integrity and accuracy of cloned sequence was confirmed by sequencing and aligning. On this basis, the prokaryotic expression vector （BL21-pET28-AtCpNifS） with the gene was constructed and the expression protein（AtCpNifS） was separated from the plasmid successfully.
作者 李亚男 冯霞 杜玉潇 陈大清
机构地区 长江大学农学院 长江大学生命科学学院
出处 《湖北农业科学》 北大核心 2010年第1期1-4,共4页 Hubei Agricultural Sciences
基金 教育部科学技术研究重点项目(207073) 湖北省教育厅重大项目(2002A0013) 荆州市科技发展计划项目(20052PB04)
关键词 拟南芥 AtCpNifS 基因克隆 原核表达 Arab idopsis thaliana A tCp NifS gene cloning prokaryotic expression
分类号 Q943.2 [生物学—植物学]
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参考文献16

  • 1SHRIFT A. Aspects of selenium metabolism in higher plants [J]. Annu Rev Plant Physiol,1969,20:475-494.
  • 2TERRY N, ZAYED A M, TARUN A S. Selenium in higher plants[J]. Annu Rev Plant Physiol,2000,51:401-432.
  • 3SORS T G,ELLIS D R,SALT D E.Selenium uptake, translocation, assimilation and metabolic fate in plants [J]. Photosynth Res,2005,86:373-389.
  • 4陈大清.植物硒同化的研究进展及其耐硒突变体的筛选[J].氨基酸杂志,2004,26(2):65-71. 被引量：22
  • 5NEUHIERL B, BOCK A. On the mechanism of selenium tolerance in elenium-accumulating plants. Purification and characterization of a specific selenocysteine methyltransferase from cultured cells of Astragalus bisulcatus [J]. Eur J Biochem, 1996,239 : 235-238.
  • 6VAN H T, ABDEL-GHANY S, HALE K L, et al. Overexpression of cystathionine-g-synthase in Indian mustard enhances selenium volatilization[J]. Planta,2003,218:71-75.
  • 7PILON-SMITS E A H, HWANG S, LYTLE C M, et al.Overexpression of ATP sulfurylase in Indian mustard leads to increased selenate uptake, reduction and tolerance [J]. Plant Physiol, 1999,119 : 123-132.
  • 8KIM Y B, GARBISU C, PICKERING I J, et al.Thioredoxin h overexpressed in barley seeds enhances selenite resistance and uptake during germination and early seedling development[J]. Planta, 2003,218: 186-191.
  • 9PILON M, OWEN J D, GARIFULLINA G F, et al.Enhanced selenium tolerance and accumulation in transgenic Arabidopsis expressing a mouse selenocysteine lyase [J].Plant Physiol, 2003,131 : 1250-1257.
  • 10GARIFULLINA G F, OWEN J D, LINDBLOM S D, et al. Expression of amouse selenocysteine lyase in Brassica juncea chloroplasts affects selenium tolerance and accumulation [J]. Physiol Plant, 2003,118 : 538-544.

二级参考文献40

  • 1Shrift A, Uirich J. 1976. Transport of selenate and selenite into Astragalus roots .[J]Plant Physiol. 44:893～896.
  • 2Cherest H, Davidian J-C, Thomas D, et al. 1997. Molecular characterization of two high affinity sulfate transporters in saccharomyces cerevisiae. [J] Generics 145:627～635.
  • 3Terry N, Zayed A.M de Souza M.P Tarun A.S Selenium in hegher plants 2000[J] Annu. Rev. Plant Physiol. Plant Mol. Biol.51:401～432.
  • 4Wilting R, Schorling S, Persson BC, Bock A. 1997, Selenoprotein synthesis in archaea: ldentification of an mRNA element of Methanococcus jannaschii probably directing selenocysteine insertion. [J]J Mol Biol, 266(4): 637～641.
  • 5Stadtman TC. 1990. Selenium biochemistry. [J]Annu. Rev. Biochem. 59:111～128.
  • 6Axley MJ, Boeck A. Stadtman TC. 1991. Catalytic properties of an Escherichia coli formate dehydrogenase mutant in which sulfur replaces selenium .[J] Proc. Natl. Acad. Sci. USA 88:8450～8454.
  • 7Anderson JW. 1993. Selenium interactions in sulfur metabolism. In Sulfur Nutrition and Assimilation in Higher Plants: Regulatory Agricultural and Environmental Aspects, ed. LJ De Kok. I Stulen, H Rennenberg, C Brouold, WERauset, pp. 49～60 The Hague, The Netherlands: SPB Academic.
  • 8Hazebrouck S, Camoin L, Zehave F, et al 2000 Substitugting selenocysteine for catalytic cysteine 41 enhances enzymatic activity of plant phosphlipid hydroperoxide glutathione peroxidase expressed in Escherichia coli. [J]The Journal of Biological Chemistry 257:28715～28721.
  • 9Novoselov S V, Rao M, Onoshko N V. et al. 2002. Selenoproteins and selenocysteine insertion system in the model plant cell system, Chlamydomonas reinhardtii.[J]The EMBO Journal vol.21 No.14 3681～3693.
  • 10Fu LH ,Wang XF , Eyal Y et al. 2002. A selenoprotein in the plant kingdom. [J] The Journal of Biological Chemistry. 277:25983～25991.

共引文献21

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二级引证文献9

湖北农业科学
2010年 第1期

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