摘要
以恩施地区五鹤续断的根尖为材料,研究了预处理时间和解离时间对其染色体制片的影响,并用此最佳条件进行了五鹤续断根尖染色体核型分析。结果表明,0.1%秋水仙素预处理2.5h、1mol/L盐酸解离30min的处理条件为最佳;五鹤续断染色体数目为18条,核型公式为2n=18=12m+4sm+2st,核型为2A型。
The roots of Wu-He Dipsacus in Enshi were used to study the effect of pre-treating time and the time of dissociation on the slide-preparing of the chromosomes.The results revealed that the pretreatment with 0.1% eolchicines 2.5h and lmol/L HCl at 60 temperature degree 30min preformed well in the sectioning. This technique was employed to determine the karyotype of Wu-He Dipsacus. The results showed that the number of chromosomes in Wu-He Dipsacus was 18 and the formula was 2n=18=12m＋4sm＋2st, and karyotype asymmetry belonged to 2A type.
出处
《湖北农业科学》
北大核心
2010年第1期129-131,共3页
Hubei Agricultural Sciences
基金
国家民委项目(08HB06)
生物资源保护与利用湖北省重点实验室(湖北民族学院)资助项目(2007025)
关键词
五鹤续断
染色体制片
核型
Wu-He Dipsacus
chromosome sectioning
karyotype
