轮叶党参叶片的组织培养研究

Study on Tissue Culture Using Leaves of Codonopsis lanceolata Benth

以轮叶党参叶片为外植体,研究了培养基及不同激素组合对愈伤组织诱导及植株再生的影响。结果表明,叶片作为外植体可以比较容易地诱导出愈伤组织,最佳诱导培养基为MS+2,4-D 1.0mg/L+6-BA 1.0mg/L+KT 0.5mg/L,最高出愈率为94.2%;诱导不定芽的最佳培养基为MS+6-BA 2.0mg/L+NAA 0.5mg/L,最高诱导率可达69.2%;根诱导的最佳培养基为MS+6-BA1.0mg/L+NAA 0.5mg/L,生根率达85.9%,建立了较为完善的轮叶党参再生体系。

The leaves were used as explants to study the influence of different hormone combinations on callus induction and plant regeneration of Codonopsis lanceolata Benth. The results showed that the optimal medium for intensive and rapid growth of callus was MS medium supplemented with 1.0mg/L 2,4-D and 2.0mg/L KT. The highest callus-forming rate reached 94.2% ;the optimal medium for regenerated seedlings was MS medium supplemented with 2.0mg/L 6-BA and 0.5mg/L NAA. The highest induction rate reached 69.2% while MS with 1.0mg/L 6-BA and 0.5mg/L NAA was the best for rooting. We have primarily established a regeneration system of Codonopsis lcmceolala Benth.