产几丁质酶真菌菌株的筛选及产酶抑菌活性的检测

Screening of fungal chitinase-producing strains and determination of enzymatic activity

目的:分离真菌菌株,筛选出几丁质酶高产菌株,检测其粗酶液对临床常见假丝酵母的抑菌作用,为抗真菌药物的开发提供研究基础。方法:利用刚果红几丁质平板法和3,5-二硝基水杨酸(DNS)法筛选出几丁质酶高产菌株,并用纸片法检测粗酶液的抑菌作用。结果:从环境中分离的47株真菌菌株中得到产几丁质酶菌株6株,其中高产菌2株(烟曲霉JLC50134和木霉JLC31235);JLC50134粗酶液对白假丝酵母、热带假丝酵母的抑制作用强于JLC31235,抑菌圈直径分别为4～5cm和约2cm;JLC31235粗酶液对克柔假丝酵母、光滑假丝酵母的抑制作用强于JLC50134,抑菌圈直径分别为约2cm和1cm。结论:烟曲霉JLC50134和木霉JLC31235是几丁质酶高产菌株,两种粗酶液对临床常见假丝酵母有抑制作用。

Objective To screen the high chitinase-producing fungi from the samples in the environment, and investigate the inhibitory effect of the enzymes from the strains on the Candida for the development of antifungal drug. Methods Congo red-chitin plates and classical DNS method were used to screen the high chitinase-producing strains, and the filter paper containing the chitinase was used to detect the inhibitory effect of the enzymes. Results 6 chitinase-producing strains were screened from 47 strains. 2 high chitinase-producing strains （Aspergillus fumigatus JLC 50134 and Trichoderma sp. JLC 31235） were obtained. The enzyme from JLC 50134 had better inhibitory effect on Candida albicans and Candida tropicalis, and the individual diameters of the inhibition zone were 4-5 cm and about 2 cm; while the enzyme from JLC 31235 had better inhibitory effect on Candida krusei and Candida glabrata than JLC 50134, and the individual diameters of the inhibition zone were about 2 cm and 1 cm. Conclusion High chitinase-producing strains （JLC 50134 and JLC 31235） are obtained , and they have different inhibitiory effects on Candida.