ATP及三醇组人参皂苷对失神经支配骨骼肌萎缩及运动终板的保护作用

Protective effects of ATP and panax ginseng triol set on denerved muscle atrophy and motor end plate

目的:观察ATP及三醇组人参皂苷对失神经支配骨骼肌萎缩及运动终板的保护作用,为临床神经损伤患者手术时机的选择提供依据。方法:选用成年雄性Wistar大鼠,建立坐骨神经损伤的动物模型,建模后大鼠随机分为对照组、ATP组、三醇组人参皂苷低剂量(25mg.kg-1)组和三醇组人参皂苷高剂量组(50mg.kg-1),术后各组分别腹腔注射生理盐水、0.1mg.d-1腓肠肌内注射ATP及低、高剂量三醇组人参皂甙,给药4周,于术后2、4、6、8、12、16、20和24周取右小腿腓肠肌,测量腓肠肌湿重、肌细胞直径、肌细胞截面积及观察运动终板形态。结果:失神经支配后骨骼肌湿重、肌细胞直径及截面积均下降,神经损伤16周内,ATP组、三醇组低剂量组及三醇组高剂量组与对照组比较差异有显著性(P<0.01)。神经损伤20周时,ATP组与对照组比较差异无显著性,三醇组低剂量组及三醇组高剂量组与对照组比较差异有显著性(P<0.05);神经损伤24周时各组间比较差异无显著性。运动终板4周内改变不明显,此后逐渐失去正常的形态,形成神经丝,至神经损伤后16周,神经丝的数量开始下降。ATP组和三醇组人参皂苷组相同时间点神经丝的数量较对照组增多。结论:ATP及三醇组人参皂苷可减缓失神经肌萎缩,提示伴有神经损伤的外伤患者临床应尽早行手术治疗以修复神经。

Objective To observe the protective effects of ATP and panax ginseng triol set on the denerved muscle atrophy and motor end plate, and provide basis for the selection of operation opportunity in patients with never injury. Methods Adult male Wistar rats were used. The model of denervated gastrocnemius muscle was established by cutting off the sciatic nerve. The rats were randomly divided into control group, ATP group, low dose panax ginseng triol set （25 mg ·kg^-1 ） group and high dose panax ginseng triol set （50 mg · kg^-1 ） group. During four weeks after operation, ATP was injected into gastrocnemius and the panax ginseng triol set was injected into belly. The muscle wet weight, diameter of myocytes, cross section area of myocytes and the morphological changes of motor end plate were measured 2, 4, 8, 8, 12, 16, 20, and 24 weeks after operation. Results After nerve injury, the muscle wet weight, diameter of myoeytes, cross section area of myocytes were decreased. During 16 weeks after nerve in）ury, there were significant differences between ATP group , low close panax ginseng triol set group, high dose panax ginseng triol set group and control group （P〈0.01）. At 20 weeks after nerve injury, there was no difference between ATP group and control group, but there were differences between low dose panax ginseng triol set group, high dose panax ginseng triol set group and control group （P〈0.05）. At 24 weeks after nerve injury, there were no differences between four groups. There were no significant changes of motor end plate during 4 weeks after nerve injury. Four weeks later, the motor end plate lose its normal morphous and changed into neurofilaments. Until 16 weeks after operation, the neurofilaments began to decrease. The number of neurofilaments in ATP and panax ginseng triol set groups was more than that in control group. Conclusion The denerved muscle atrophy and motor end plate cataplasia could be deferred by using ATP and panax ginseng triol set.