舒肝解郁灵对抑郁大鼠行为及血浆ACTH及血清T_3、T_4、TSH和rT_3水平的影响

Effects of Shugan Jieyu Panacea on behavior and levels of ACTH in plasma and T_3,T_4,TSH and rT_3 in serum in depression rats

目的:通过检测舒肝解郁灵(SJP)对抑郁大鼠行为及血浆ACTH和血清T3、T4、TSH和rT3水平的影响,探讨其抗抑郁作用及其机制。方法:采用孤养和慢性轻度不可预知的应激刺激随机组合方法建立稳定的大鼠抑郁模型,设正常对照组、抑郁模型组、舒肝解郁灵小、中、大剂量组及阳性药物盐酸氟西汀组,连续给药21d,定期测定各组大鼠体质量、糖水消耗量及行为学改变,采用放免法测定大鼠血浆ACTH、血清T3、T4、TSH和rT3水平。结果:与正常对照组比较,模型组大鼠体质量下降(P<0.01),运动次数减少(P<0.01),糖水消耗量减少(P<0.01)。与抑郁模型组比较,给药后SJP小、中、大剂量组大鼠体质量升高(P<0.01),行为活动次数增加(P<0.05或P<0.01),糖水消耗量增加(P<0.01),ACTH、T4和rT3含量明显降低(P<0.05或P<0.01),T3含量明显升高(P<0.05),与盐酸氟西汀组比较差异无显著性。结论:SJP可以明显改善大鼠抑郁状态,其机制可能与SJP调节HPA轴及HPT轴功能有关。

Objective To investigate the effeets of Shugan Jieyu Panacea （SJP） on behavior and the levels of ACTH in plasma and T3, T4 , TSH, rT3 in serum in depression model rats and explore the mechanism. Methods The model rats were lonely fed and received chronic moderate intensive unpredictable stimulation. Normal control group, depressed model group, high dosage SJP group, middle dosage SJP group, low dosage SJP group and fluoxetine group were set up. Defferent drugs were used in various groups for 21 d, then the body mass, sugar consumption and the behavior changes of the rats were determined and the levels of ACTH in plasma and T3, T4, TSH, rT3 in serum were detected with radioimmunoassay. Results Compared with normal group, the body mass was decreased （P〈0.01）, the scores of movement and sugar consumption were decreased （P〈0.01） in model group. Compared with model group , the body mass was increased （P〈0. 01）, the scores of movement and sugar consumption were increased （P〈0. 01）, the levels of ACTH, T4, rT3 markedly decreased （P〈0. 05 or P〈0.01） and the level of T3 was increased （P〈0.05） in high, middle, low dosage SJP groups after treatment. At the same time, there was no obvious difference between SJP groups and fluoxetine groups. Oonclusion SJP can significantly improve the depression in rats, its mechanism may be connected with adjusting the function of HPAA and HPTA.