多环芳烃不同程度污染城市人群尿中多环芳烃代谢产物水平的检测及其意义

Determination of urinary PAHs metabolites of population in polluted cities with PAHs and significance

目的:研究多环芳烃不同程度污染城市人群尿中多环芳烃代谢产物1-羟芘葡糖苷酸(1-OHPG)和2-萘酚(2-naphthol)的水平,阐明1-OHPG和2-萘酚作为评价多环芳烃污染及对人群健康危害指标的可行性。方法:采用随机整群抽样方法,在多环芳烃(PAHs)严重污染(大同)、中等污染(长春)和较轻污染(昆明)的城市,分别对60名儿童及其家长进行问卷调查,并测定尿中多环芳烃代谢产物1-OHPG和2-萘酚的水平。结果:大同市学生尿中1-OHPG水平[(10.94±6.58)μmol.mol-1肌酐]和家长尿中1-OHPG水平[(5.87±6.87)μmol.mol-1肌酐]高于长春学生[(6.33±8.54)μmol.mol-1肌酐]和家长[(4.60±4.08)μmol.mol-1肌酐],也高于昆明学生[(1.90±3.57)μmol.mol-1肌酐]和家长[(1.62±2.44)μmol.mol-1肌酐](P<0.01),长春市学生和家长尿中1-OHPG水平高于昆明市学生和家长尿中1-OHPG水平(P<0.01)。大同、长春、昆明三城市学生之间和家长之间尿中2-萘酚水平比较差异均无显著性(P>0.05)。结论:尿中1-OHPG水平能较好地反映人群接触PAHs污染的状况,可作为评价PAHs污染对人群健康损害的生物指标。

Objective To study the levels of the population-based urinary polycyclic aromatic hydrocarbons （PAHs） metabolites, 1-hydroxypyrene glucuronide （I-OHPG） and 2-naphthol, in polluted cities with different levels of PAHs, and to clarify the feasibility of 1-OHPG and 2-naphthol as an evaluation of PAHs pollution and health hazards on the population. Methods 60 students and their parents from the three cities （Datong, Changchun and Kunming） were chosen via cluster random sampling method to fill in questionnaire and their urinary 1-OHPG and 2-naphthol levels were measured. Results The levels of urinary 1-OHPG in students （10. 94 μmol·mol^-1 ± 6.58 μmol·mol^-1 creatinine） and parents （5.87 μmol·mol^-1±6.87μmol·mol^-1 creatinine） in Datong were higher than those in Changchun （students= 6.33 μmol·mol^-1 ±8.54 μmol·mol^-1 creatinine; parents= 4.60 μmol·mol^-1± 4.08 μmol·mol^-1 creatinine） and Kunming （ students, 1.90 μmol·mol^-1± 3.57 μmol·mol^-1 creatinine; parents： 1.62 μmol·mol^-1±2.44 μmol·mol^-1 ereatinine） （P〈0.01）. The levels of urinary 1- OHPG in students and parents in Changchun were higher than those in Kunming （P〈0.01）. Moreover, in the children and their parents, there were no significant differences of the urinary 2 naphthol levels between three cities （P~〉0.05） . Conclusion The urinary 1 OHPG level could properly represent the pollution condition of PAHs. It could be used as a biomarker to evaluate the population harm of PAHs pollution.