摘要
对巴西橡胶树胶乳C-乳清蛋白质双向电泳技术的各个条件,包括蛋白样品提取步骤、蛋白上样量、适宜胶条的选择、等电聚焦程序、聚丙烯酰胺凝胶电泳的凝胶浓度以及凝胶染色方法等进行了研究,确定了一套适合C-乳清蛋白质组分析的双向电泳的技术方法。
A 2-dimensional electrophoresis technology were developed for latex C-serum of rubber tree (Hevea brasiliensis) , including the method of protein extraction, loading volume of protein, selection of immobilized pH gradients, protocol of iso-electric focusing, gel density of SDS-PAGE and the visualization of protein spots in gel.
出处
《热带作物学报》
CSCD
2009年第11期1618-1623,共6页
Chinese Journal of Tropical Crops
基金
农业部热带作物栽培生理学重点开放实验室开放课题(No.KLOFO601)资助