10种热带牧草根际高效解磷细菌的筛选及其培养条件

Screening of High-efficiency Phosphate Solubilizing Bacteria from Rhizosphere of Tropical Pasture and Their Optimal Cultural Conditions

从热研2号柱花草等10种不同牧草中筛选出9株无机解磷细菌及6株有机磷细菌,并利用平板溶磷圈法、钼锑抗比色法对分离得到的细菌进行测定,最后得到解磷效果最好的解磷细菌株,牧草根际解无机磷细菌解磷能力最高达216.57mg/L。选定2株具有代表性的解无机磷细菌GI2-6(Bacillus spp.)和GI9-4-3(Serratia spp.),通过单因子试验对其解磷特性和培养条件进行研究,结果表明:当解磷细菌GI2-6以蔗糖为碳源、KNO3为氮源、pH为7.5、温度为30℃,解磷细菌GI9-4-3以葡萄糖为碳源、KNO3为氮源、pH为7.0、温度为30℃时,两者的菌体量最大,解磷效果最好。

Fifteen strains of phosphate-solubilizing bacteria were isolated from the rhizospheres of 10 species of tropical pasture like Stylosanthes ＇Reyan 2＇, etc, and were identified by using plate P- solubilizing circle method and the Mo-Sn-Vc method. Two strains, GI2-6（Bacillus spp.） and GI9-4-3 （Serratia spp.）, which possess strong inorganic phosphorus solubilizling ability were selected from these 15 strains. A single-factor design was arranged to study the phosphorus dissolving ability and the optimal cultural conditions of these 2 strains. The results showed that these 2 strains produced the largest colonies and best phosphorus dissolution when sucrose was used as carbon source and KNO3 as nitrogen source, the initial pH 7.5 and the culture temperature 30 ℃ for the strain GI2-6, and glucose as carbon source, KNO3 as the nitrogen source and the initial pH 7.0 and the culture temperature 30℃ for the strain GI9-4-3.