摘要
采用正交设计方法,研究橡胶树品种云研77-2茎段快繁试验中NAA、KT、6-BA3种激素对茎芽诱导的影响。结果表明,培养基中添加6-BA对茎芽诱导起主导性的作用。橡胶树云研77-2茎芽诱导最适培养基为MS+2.0mg/L6-BA+0.1mg/LNAA+0～0.5mg/LKT。诱导生根试验中,培养基为1/2MS+0.5mg/LIBA的生根率可达66.7%。叶片剪去1/2的自根无性系进行移栽的植株平均成活率为43.6%。
Three hormones, NAA, KT and 6-BA, were used to induce stem segments for rapid propagation of clone Yunyan77-2, and an orthogonal design was arranged. The results showed that the medium in the presence of 8-BA had a dominant role in inducing shoots from the stem segments. The optimal shoot inducing medium was MS ＋ 2.0 mg/L6-BA ＋ 0.1 mg/L NAA ＋ （0-0.5）mg/L KT. The root inducing medium of 1/2MS ＋ 0.5 mg/L IBA produced a rooting rate of 66.7%. The self-rooted plantlets with 1/2 leaves eut had a survival rate of 43.6% after transplanted.
出处
《热带作物学报》
CSCD
2009年第11期1637-1640,共4页
Chinese Journal of Tropical Crops
关键词
橡胶树
组织培养
正交设计
rubber tree
tissue culture
orthogonal design.
