晕船易感大鼠脑干总抗氧化能力、超氧化物歧化酶及活性氧的活性测定

Measurement of total antioxidative capacity,superoxde dismutase and reactive oxygen species activity of brain stem from seasickness susceptible rats

目的:观察晕船易感大鼠脑干总抗氧化能力(total antioxidative capacity,T-AOC)、超氧化物歧化酶(superoxidedismutase,SOD)及活性氧(reactive oxygen species,ROS)的活性变化,探讨氧化应激在晕船易感性发生中的作用。方法:根据模拟晕船刺激后异嗜高岭土情况将大鼠分为易感组、晕船组、不晕组和对照组,分别用比色法、黄嘌呤氧化酶法和Fenton法检测各组大鼠脑干T-AOC、SOD及ROS的活性。结果:易感组大鼠脑干T-AOC和SOD较不晕组和对照组均显著降低(P<0.01),晕船组大鼠T-AOC和SOD水平较易感组略有升高但仍低于对照组和不晕组(P<0.05),不晕组较对照组T-AOC及SOD水平也略有下降,但差异无统计学意义(P>0.05)。ROS的变化趋势与T-AOC、SOD相反。结论:晕船易感性发生时大鼠脑干ROS活性升高,T-AOC和SOD活性降低,提示氧化应激在晕船易感性的发生和发展中起到了非常重要的作用。

Objective： To study the activity changes of total antioxidative capacity （T-AOC）, superoxde dismutase（SOD） and reactive oxygen species（ROS） of brain stem from seasickness susceptible rats, and explore the function of oxidative stress in seasickness susceptibility. Methods： According to kaolin intakes after seasickness motivation, rats were divided into seasickness susceptible group, seasickness group, non- seasickness group and control group. T- AOC, SOD and ROS were measured by colorimetry, xan- thine and fenton methods respectively. Results： T- AOC and SOD in seasickness susceptible group decreased dramaticaUy than those of non-seasickness group and control group（P〈0.01）. T-AO and SOD of seasickness group were slightly higher than seasickness susceptible group but lower than those of control group and non-seasickness group（P〈0.05）. T- AOC and SOD of non-seasickness group were lower than those of the control group but without statistic significance（P〉 0.05 ）. ROS changed the opposite way of T- AOC and SOD. Conclusion： The anti - oxidative capability of rat brain stem decreases during the procedure of seasickness susceptibility while reactive oxidative species increases, which suggests that oxidative stress plays an important role in the development of seasickness susceptibility.