三氯乙烯致敏豚鼠血清中肿瘤坏死因子-α和白细胞介素-1β水平的变化

Changes of serum TNF-α and IL-1β levels in guinea pig sensitized with trichloroethylene

目的比较三氯乙烯(TCE)致敏豚鼠和未致敏豚鼠血清中白细胞介素(IL)-1β和肿瘤坏死因子(TNF)-α水平。方法将豚鼠随机分为空白对照组,溶剂(橄榄油)对照组,2,4-二硝基氯苯(DNCB)阳性对照组,TCE处理组。根据豚鼠最大值试验(Guinea pig maximization test,GPMT)方法处理豚鼠。按照《化学品毒性鉴定技术规范》的评分标准对动物的皮肤反应进行评分,评分≥1的判为致敏。在末次激发后24 h和72 h分2批采血,用聚合酶链反应试剂盒测定血清中TNF-α、和IL-1β的含量。结果DNCB组致敏率为100%,TCE组致敏率为62.1%。DNCB阳性对照组和TCE致敏组TNF-α和IL-1β水平与溶剂对照组相比明显升高(P<0.05)。在24 h及72 h 2个时点,TCE致敏动物TNF-α和IL-1β水平比相应的未致敏组高,差异有显著性(P<0.05)。结论在TCE诱导的致敏豚鼠血清中TNF-α和IL-1β水平升高。

Objective To detect serum IL-1β and TNF-α levels in guinea pigs sensitized with trichloroethylene（TCE）. Methods Guinea pigs were randomly divided into blank control groups, solvent （olive oil） control groups, DNCB positive control groups and TCE treatment groups. Guinea pigs were treated with adopting guinea pig maximization test （ GPMT）. According to ＂Chemical Toxicity Appraisal Technology Standard＂ ,the animals were judged to be sensitized when the skin reaction score was ≥ 1. Bloods were collected 24 or 72 hours after last challenge. Serum IL-1β and TNF-α levels were detected by ELISA Kits. Resuits Sensitization rates in DNCB positive control groups were 100% while 62.1% in TCE treatment groups. Compared with the solvent control groups, serum TNF-α and IL-1β levels in DNCB positive control groups and TCE sensitized groups significantly elevated （ P 〈 0.05 ）. At the point of 24 hours and 72 hours, serum TNF-α and IL-1β levels in TCE sensitized groups were higher than those in non-sensitized groups （ P 〈 0. 05 ）. Conclusion Serum TNF-α and IL-1β levels were elevated in TCE sensitized guinea pigs.