解偶联蛋白2在老年大鼠免疫器官中的表达

The Expression of UCP2 in the Immune Organs of Old - aged Rats

目的探讨解偶联蛋白2（uncoupling protein2，UCP2）在老年大鼠免疫器官中的表达。方法健康雄性SD大鼠30只，按年龄分为新生组、成年组和老年组，实验室适应性喂养7d后断头处死，分别取各组大鼠脾、胸腺组织测定丙二醛（malondialdehyde，MDA）、谷胱甘肽（glutathione，GSH）；通过免疫组织化学观察脾、胸腺组织中UCP，蛋白的表达与分布。结果老年组大鼠脾、胸腺组织中MDA含量显著高于幼年组和成年组（P〈0．01）；GSH含量在老年组大鼠脾组织中显著低于幼年组和成年组（P〈0．01），在老年组大鼠胸腺组织中显著低于幼年组（P〈0．01）；UCP，蛋白在老年组大鼠脾、胸腺实质细胞内表达较多，在幼年组、成年组大鼠脾、胸腺实质内表达较少（几乎无表达）。结论老年大鼠脾、胸腺MDA含量升高，GSH含量下降，提示老年大鼠脾、胸腺内活性氧（reactive oxygen species，ROS）水平较高，并引起UCP2表达增多，UCP2可能对延缓脾、胸腺细胞的衰老和增强免疫功能起重要作用。

Objective To discuss the expression of UCP2 in the immune organs of old - aged rats. Methods Thirty healthy male SD rats were chosen and divided into three groups： young group, adult group and old - age group fed for 7 days and then beheaded. These rats＇ spleen and thymus tissues were taken out respectively to test the content of malondialdehyde （ MDA, glutathione （GSH） and the expression and distribution of UCP2 in the spleen and thymus tis- sues was observed by chemical coloration in immuagte. Results In the old - aged group, the MDA content in the spleen and thymus tissues was remarkably higher than that in the young group and adult group （p 〈 0.011 . On the contrary, the GSH content in the old - aged rats＇ spleen tissue was remarkably lower than that in the young group and adult group （p 〈 0.01 ）, and in the old - aged rats＇ thymus tissue, the GSH content was remarkably lower than that in the young group （p 〈 0.01 ） . The expression of UCP2 in the old - aged rats＇ spleen and thymus substantial cells turned out to be much more than in the young and adult rats＇ substantial cells where the expression nearly can＇ t be observed. Conclu- sion The content of MDA rises while the content of GSH descends in the spleen and thymus of old - aged rats. The result suggests that the level of （ reactive oxygen species, ROS） is relatively higher and causes the increase of UCP2 expression. UCP2 may play an important role in staving off the aging of spleen and thymus ceils and improving the immune system.