基于“单碱基延伸和酶放大系统”的SNP漏声表面波生物传感器的实验研究

Single base extension and enzyme amplification system based single nucleotide polymorphism leaky surface acoustic wave biosensor

目的探讨漏声表面波生物传感器检测单核苷酸多态性(single nucleotide polymorphism,SNP)的方法。方法分别在漏声表面波生物传感器反应体系加入2条仅存在一个碱基不同(A/G)的靶序列,与固定的探针杂交。利用酶生物信号放大系统,结合单碱基延伸技术,观察其杂交信号的改变。结果靶位点为完全匹配的A时,相位下降(6.47°±0.42°);而靶位点为错配的G时,相位下降仅为(0.77°±0.25°),两者差异有统计学意义(P<0.01)。结论成功建立了漏声表面波生物传感器检测SNP的新方法。

Objective To study how to use the leaky surface acoustic wave （LSAW） biosensor to detect single nucleotidc polymorphism （SNP）. Methods Two target sequences with a base difference （A/G） were added onto the LSAW biosensor reaction system and hybridized with corresponding probes. The enzyme signal amplification system was used in combination with single base extension （SBE） technology to observe changes in hybridization signals. Results The corresponding phase of targets was decreased to 6.47 ° ± 0.42° and 0.77° ± 0.25°, respectively, when the target point completely matched A and mismatched G （P 〈 0.01 ）. Conclusion A new method for the detection of SNP with a LSAW biosensor has been successfully established.