米托蒽醌对人乳腺癌细胞MCF-7增殖的影响

Effects of mitoxantrone on the proliferation of MCF-7 cell lines

目的:观察米托蒽醌对人乳腺癌细胞MCF-7的影响。方法:台盼蓝拒染观察药物对细胞的不良反应,流式细胞术观察药物对细胞周期的影响。结果:米托蒽醌对MCF-7细胞具有明显的增殖抑制作用,呈一定的时效性;药物作用MCF-7 48h后能引起其细胞周期发生变化。结论:米托蒽醌能明显地抑制MCF-7细胞的增殖,并能引起其细胞周期变化。

Objective： To observe the effect of mitoxantrone on MCF - 7 cell lines. Methods ： The effects of mitoxantrone on the proliferation of MCF - 7 cell lines were detected by Trypan blue dye exclusion assay, the changes of cell cycle were analyzed by flow cytometry （FCM）. Results： Mitoxantrone had strong cytotoxicity to MCF - 7 cell lines with dependence of time. After treated with mitoxantrone for 48h, abnormality of cell cycles could be induced in MCF -7 cells. Conclusion： Mitoxantrone could inhibit the proliferation of MCF -7 cell lines and induce the abnormality of cell cycles.