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三维立体培养中H460细胞MMP-2、MMP-9的表达

MMP-2 and MMP-9 expressions of H460 cells in three-dimensional culture
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摘要 目的:建立肺癌细胞的三维立体培养模型,测定肺癌细胞在三维立体培养中的基质金属蛋白酶(MMPs)的表达,研究MMPs在肺癌侵袭与转移中的作用。方法:采用非小细胞肺癌H460细胞系建立肺癌细胞三维立体培养模型,观察癌细胞的生长,同时设置平面培养对照组,用明胶酶谱法测定两组不同时间点MMP-2、MMP-9。结果:H460细胞在立体胶原内生长良好,分泌有MMP-2、MMP-9,且分泌量多于平面培养组。结论:三维立体培养肺癌细胞能清晰地表达MMPs,是研究MMPs在癌细胞的侵袭与转移作用机制的一种可行方法。 Objective： To determine the expression of matrix metalloproteinases （MMPs） of non - small cell lung cancer（NSCLC） in three -dimensional culture,and the effects of MMPs in the invasion and metastasis of lung cancer. Methods： Establishing the three - dimensional model of H460 cell line in collagen gels to observe cancer cell growth by comparing to mono - layer culture. The expression of MMP - 2 and MMP - 9 was determined by gelatinase zymography. Results：Cancer cells growed well in collagen gels and secrete MMP -2 and MMP -9 more than those in mono - layer culture condition. Condusion： The model of lung cancer which expressed more MMPs is a feasible method for the study of MMPs in the invasion and metastasis of lung cancer.
作者 李继东 朱运奎 吴昌归 肖永久 薛庆亮 赵斌斌
机构地区 兰州军区兰州总医院呼吸内科 第四军医大学附属西京医院呼吸内科
出处 《现代肿瘤医学》 CAS 2010年第2期238-240,共3页 Journal of Modern Oncology
基金 甘肃省自然科科学基金资助项目(编号:3ZS051-A25-088)
关键词 立体培养 基质金属蛋白酶 H460细胞 肺恶性肿瘤 three - dimensional culture matrix metalloproteinases H460 cells lung cancer
分类号 R73-75 [医药卫生—肿瘤] R734.2 [医药卫生—肿瘤]
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参考文献11

  • 1Stephen GS, Gerard AS. One hundred years of lung cancer[ J]. Am J Respir Cri Care Med,2005 ,172 :523 - 529.
  • 2Reuven R, Yiffat K, Rivka H, et al. Carbamoylphosphonate matrix metalloproteinase inhibitors 3 : in vivo evaluation of eyclopentylcar- bamoylphosphonie acid in experimental metastasis and angiogenesis [ J ]. Clin Cancer Res,2005,11 : 3925 - 3929.
  • 3Parson SL, Waeton SA, Bromn PD, et al. Matrix metalloproteinases [ J ]. Br J Surg, 1997,84 : 160.
  • 4Kim JB. Three - dimensional tissue culture models in cancer biology[J]. Semin Cancer Biol,2005,15:365 -377.
  • 5Carterson AJ, Bentrup KH, Ott CM, et al. A549 lung epithelial cells grown as three - dimensional aggregates : alternative tissue culture model for pseudomonas aeruginosa pathogenesis [ J ]. Infection and Immunity,2005,2 : 1129 - 1140.
  • 6Wang R, Xu J, Juliette L, et al. Three - dimensional co - culture models to study prostate cancer growth, progression, and metastasis to bone [ J ]. Semin Cancer Biol,2005,15 (5) :353 - 364.
  • 7Thomas P, Khokha R. Diferential expression of matrix metalloproteinases and their inhibitors in non - small cell lung cancer [ J ]. J Pathol.2000,190 : 150 - 156.
  • 8张海泉,周毅,敖祥生,黄星,刘汉东.EMMPRIN、MT1-MMP及MMP2在人脑胶质瘤中的表达及意义[J].现代肿瘤医学,2009,17(3):442-446. 被引量：3
  • 9杨大运,丁翠敏,孙志学,金普乐,王平.基质金属蛋白酶-2及其抑制剂在肺癌侵袭转移中作用的研究[J].国外医学（呼吸系统分册）,2005,25(6):407-409. 被引量：5
  • 10Zucker S, Hymowitz M, Rollo EE, et al. Tumorigenic potential of extracellar matrix metalloproteinase inducer [ J ]. Am J Pathol, 2001,158 : 1921 - 1928.

二级参考文献30

  • 1李为民,陈文彬,徐丹,朱辉,魏大鹏,周清华.纤维支气管镜刷检细胞核内不均一核糖核蛋白B_1染色诊断肺癌价值的研究[J].中国呼吸与危重监护杂志,2004,3(4):229-232. 被引量：14
  • 2Nakada M, Kita D, Futami K, et al. Roles of membrane type - 1 matrix metalloproteinase and tissue inhibitor of metalloproteinases 2 in invasion and dissemination of human malignant glioma [ J]. J Neurosurg,2001,94 ( 3 ) :464 - 473.
  • 3Sameshima T, Nabeshima K, Bryan P, et al. Expression of EMM- PRIN (CD147), a cell surface inducer of matrix metalloproteinases, in normal human brain and gliomas [ J ]. Int J Cancer,2000,88 (1) :21-27.
  • 4Dalberg K, Eriksson E, Enberg U,et al. Gelatinase A, membrane type 1 matrix metalloproteinase, and extracellular matrix metallo- proteinase inducer mRNA expression: correlation with invasive growth of breast cancer[ J]. World J Surg,2B00,24 (3) :334 - 340.
  • 5Ahlan J, Enberg U, Larsson C, et al. Malignant fibrous histiocytoma,aggressive fibromatosis and benign fibrous tumors express mRNA for the metalloproteinase inducer EMMPRIN and the metalloproteinases MMP -2 and MT1 - MMP[J]. J Sarcoma,2001,5 :143 -149.
  • 6Suzuki S, Sato M, Senoo H, et al. Direct cell - cell interaction en- hances pro - MMP - 2 production and activation in co - culture of laryngeal cancer ceils and fibroblasts: involvement of EMMPRIN and MT1 - MMP[ J ]. J Exp Cell Res,2004,293 (2) :259 - 266.
  • 7Sameshima T, Nabeshima K, Bryan P, et al. Glioma cell extraceUu- lar matrix metalloproteinase inducer(EMMPRIN) (CD147) stimulates production of membrane - type matrix metalloproteinases andactivated gelatinase A in co - cultures with brain - derived broblasts[ J]. J Cancer Lett,2000,157(2) :177 - 184.
  • 8Sameshima T, Nabeshima K, Bryan P, et al. Correlation of emmprin expression in vascular endothehal cells with blood - brain - barrier function : a study using magnetic resonance imaging enhanced by Gd - DTPA and immunohistochemistry in brain tumors[ J]. J Virchows Arch ,2003,442 (6) :577 - 584.
  • 9American Joint Committee on Cancer (AICC). Lung. In: Fleming ID, Cooper JS, Hensen DE, eds. Cancer Staging manual. 5th rd. Philadephia: Lippincott-raven, 1997; 127.
  • 10Flischhacker M, Beinert T, Ermitsch M, et al. Heterogeneous nuclear ribonucleoprotein B1 as a new marker of early detection for human lung cancers. Cancer Res, 1999 ;59:1404～1407.

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现代肿瘤医学
2010年 第2期

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