河南汉族人群血小板膜糖蛋白IaC_(807)T基因多态性及其对血小板聚集功能的影响

Polymorphism of Platelet Glycoprotein IaC_(807)T Gene in Han Population of Henan Province and Its Effect on Platelet Aggregation

目的:探讨河南汉族人群血小板膜糖蛋白(GP)IaC807T基因多态性及其对血小板聚集功能的影响。方法:120例河南汉族脑梗死患者为缺血组,113例健康体检者为对照组,PCR-RELP法检测2组GPIaC807T基因多态性,体外胶原诱导法检测2组血小板聚集功能。结果:河南汉族人群GPIa基因第807位存在2种等位基因(C和T)和3种基因型(C/C、C/T、T/T);缺血组T807等位基因频率高于对照组(P<0.05);GPIaT等位基因携带者较C等位基因携带者体外胶原诱导下血小板达30%聚集前的延迟期缩短(P<0.05);缺血组T基因携带者血小板达30%聚集率前的延迟期较对照组缩短(P<0.05);血小板最大聚集率差异无统计学意义。结论:血小板膜GPI-aT807等位基因可能是河南汉族脑梗死患者的遗传危险因素,作用机制可能与血小板聚集功能的迅速启动有关。

Objective： To investigate the polymorphism of the platelet glycoprotein IaC807T gene in Han poplulation of Henan province and its effect on platelet aggregation. Methods： Platelet glycoprotein IaC807T gene polymorphism in 120 patients with cerebral infarction （cerebral infarction group） and 113 healthy volunteers （control group） from Han population of Henan province have been detected using PCR-RFLP technique. The technique of collagen type I-inducing platelet aggregation was used to detect the platelet aggregation of two groups. Results： There were two alleles （C and T） and 3 genotypes （C/C C/T T/T）. The frequency of GPIa T allele was significantly higher in the cerebral infarction group than that in the control group （P〈0. 05）. The lag time before 30% platelet aggregation was significantly shorter in the subjects with T allele than those with C allele in two groups （P〈 0.05） and the lag time before 30% platelet aggregation of subjects with T allele in cerebral infarction group was significantly shorter than those in the controls. However, there was no significant difference of the maximal rate of platelet aggregation between them. Conclusion： The platelet collagen receptor GPIaT807 allele might be a risk factor for cerebral infarction in patients in Henan province and the rapid initiation of collagen-induced platelet aggregation may be one of the mechanisms.