大鼠小体积肝移植物再灌注早期EGR-1的表达及其意义

Expression and role of early growth response(Egr-1) in reperfusion injury of small-for-size graft in rat liver transplantation

目的探讨早期生长因子(EGR-1)在大鼠小体积肝移植物早期损伤中的表达及意义。方法雄性SD大鼠随机分成3组:假手术组(SO组),小体积肝移植物组(30%供肝组),全肝移植物组(100%供肝组)。采用两袖套法建立肝移植模型,比较再灌注24 h内3组肝功能指标变化、肝脏组织病理学改变及肝移植物内EGR-1和内皮素-1(ET-1)表达的变化。结果假手术组AST水平明显低于其余两组(P<0.01),而100%供肝组AST水平低于30%供肝组,其中6,24 h时点上,两者差异具有统计学意义(P<0.01)。光镜检查显示24 h 30%供肝的肝窦明显扩张,肝窦内衬细胞崩解、分离。假手术组未见EGR-1和ET-1表达,30%供肝组EGR-1和ET-1表达明显强于100%供肝组。结论小体积肝移植物早期损伤与EGR-1高表达及受其调控的ET-1表达上调有关。

Objective To investigate the expression and role of early growth response（EGR-1） in early injury of small-for-size graft in rat liver transplantation.Methods Male Sprague-Dawley rats were randomly divided into three groups： Sham operation group（SO group）;small-for-size graft group（30%graft group）;whole graft group（100%graft group）.The models of rat small-for-size liver transplantation were set up by two-cuff technique.Changes of AST in serum,pathology of liver,expression of EGR-1 and endothelin（ET）-1 in liver of three groups within 24 hours after reperfusion were compared.Results AST of SO group was significantly lower than that of the other two groups（P0.01）,while AST of 100% graft group was lower than that of 30% graft group,especially at 6h and 24h after reperfusion（P0.01）.Apparent dilation of the sinusoid with disintegration and detachment of sinusoidal lining cells were observed in 30%graft group at 24h after reperfusion.There was no expression of EGR-1 and ET-1 in SO group,whereas expression of these two factors in 30%graft group were significantly higher than that in 100% graft group within 24 hours after reperfusion.Conclusions Small-for-size graft injury was related to early over-expression of EGR-1 associated with upregulation of ET-1.