姜黄素对肝缺血再灌注损伤保护作用的研究

Research on the protective effects of curcumin against liver ischemia reperfusion injury in liver autotransplantation in rats

目的探讨姜黄素对自体肝移植大鼠肝脏缺血再灌注损伤(IRI)的保护作用及其可能机制。方法54只SD雄性大鼠随机分为假手术(SO)组、自体肝移植模型姜黄素预处理(CU)组以及自体肝移植模型溶剂对照(CM)组,术后或再灌注2,6,24 h每组分别处死6只大鼠进行谷丙转氨酶(ALT)、谷草转氨酶(AST)、髓过氧化物酶(Myeloperoxidase MPO)的含量检测。结果血清ALT及AST水平,CM组及CU组均较SO组明显升高,但CM组又明显高于CU组;MPO在SO组含量明显低于CM组和CU组,而CU组又显著低于CM组。结论姜黄素对自体肝移植大鼠肝缺血再灌注损伤有保护作用,其机制可能与减轻中性粒细胞浸润有关。

Objective To explore the possible mechanism of protective effects of curcumin against the ischemia reperfusion injury（IRI） in liver autotransplantation in rats.Methods A total of 54 male SD rats were randomly divided into three groups：curcumin preconditioning（CU） group,0.5% sodium carboxymethycellulose preconditioning（CM） group,and the sham operation（SO） group.In CU and CM groups liver autotransplantation model were constructed.Then,6 rats of each group were executed at 2,6,24 h after operation or liver reperfusion,respectively.Plasma samples were collected for ALT and AST test and liver tissues were harvested to detect the content of the MPO.Results The serum level of ALT and AST in CU group,and CM group was markedly elevated compared to SO group,but in CU group was significantly lower than CM group at 2,6,24 h after reperfusion.Compared with SO group,the content of MPO was decreased significantly in CM group and CU group,and was significantly reduced in CU group compared to CM group.Conclusions Curcumin preconditioning can attenuate liver IRI and the mechanism may be associated the decreasa of neutrophil infiltration.