摘要
目的探讨外周血中NK细胞活化性受体NKG2D及其可溶性配体MHC-I链相关蛋白A(sMICA)与原发性肝细胞癌(HCC)发生发展的关系,为HCC免疫治疗提供理论依据。方法随机选取HCC患者40例,术前收集其外周血,流式细胞术检测NKG2D的表达,ELISA检测血清中sMICA的水平,并与20例健康对照组比较,分析sMICA与HCC临床病理因素的关系。结果HCC组NKG2D的表达为(71.97±4.96)%,明显低于对照组的(91.45±3.16)%,两组之间差异有统计学意义(P<0.05)。肝癌sMICA水平较对照组显著升高(中位值191.3 pg/mL vs.74.9 pg/mL,P<0.05)。血清中sMICA水平高低与肝癌TNM分期和是否合并门静脉癌栓有关(P<0.05)。Spearman等级相关分析显示,sMICA水平与NKG2D的表达率呈显著负相关(r=-0.591,P<0.05)。结论HCC组NKG2D表达下降;其血清中sMICA水平与HCC的发生发展有密切关系,可能与NKG2D-sMICA参与了HCC免疫逃逸机制有关。
Objective To investigate the natural killer cell stimulatory receptor NKG2D and its soluble ligand MICA(sMICA) expression in peripheral blood in hepatocellular carcinoma(HCC).Methods Blood samples of 40 patients and 20 healthy persons were collected to detect NKG2D by flow cytometry analysis.Serum levels of sMICA were determined by ELISA.The association of sMICA levels wth clinical features were analyzed.Results The expression of NKG2D in HCC(71.97±4.96)% was significantly lower than that in healthy group(91.45±3.16)%.The serum levels of sMICA was found to be significantly elevated in HCC compared with heathy subjects(median,191.3pg/mL vs.74.9pg/mL,P0.05).The levels of sMICA correlated significantly with presence of tumor thrombi in portal vein and advanced clinical tumor-node-metastasis(P0.05).Spearman rank correlation displayed that the expression of NKG2D was negatively correlated with the levels of sMICA(r=-0.591,P0.05).Conclusions The expression of NKG2D in HCC is significantly decreased.The levels of sMICA correlates significantly with progression of HCC.NKG2D-sMICA may play a critical role in tumor immunological escape in HCC,and may contribute to HCC progression.
出处
《中国普通外科杂志》
CAS
CSCD
北大核心
2010年第1期57-60,共4页
China Journal of General Surgery