牛磺酸对锰中毒大鼠学习记忆的影响

Effects of taurine on the learning and memory capacities in Manganese poisoned rats

目的研究牛磺酸对锰致大鼠学习记忆能力减退的预防、治疗效应。方法选取100只体质量相近的无特定病原体(SPF)级雄性SD大鼠随机分为5组,A组:24周对照组;B组:24周牛磺酸治疗组;C组:12周染锰组;D组:12周对照组;E组:12周牛磺酸预防组。给予相应的干预措施。干预结束后,每组大鼠进行为期1周的定位航行实验测定大鼠学习记忆能力。结果大鼠平均逃避潜伏期呈整体下降趋势。在进行为期2d的适应性训练后,各组大鼠的平均逃避潜伏期在第3天、第4天差异有统计学意义,C组(29,25)s明显长于D组(17,14)s和E组(16,13)s,但比A组(41,35)s短。从第5天开始,各组平均逃避潜伏期变化趋于平缓。D、E2组每天差别不大,A、B2组基本每天长于其余3组。结论染锰大鼠学习记忆能力下降,相比之下牛磺酸干预大鼠有较好的学习记忆能力,说明牛磺酸在一定程度上能拮抗锰对神经系统的毒性作用。另外年龄可能是影响大鼠测试成绩的因素。

Objective To study the protective and therapeutic effects of taurine on the learning and memory capacities in Manganese （Mn） poisoned rats. Methods One hundred healthy male SD rats in comparable weights were randomized into 5 groups： the 24-week control group （Group A）, 24-week taurine treatment group （Group B）, 12-week Mn-stained group （Group C）, 12-week control group （Group D） and 12-week taurine prevention group （Group E） to receive interventions accordingly. After the interventions, each rat was assessed in a one-week study with navigation test for learning and memory capacities. Results The mean escape latency showed an overall downward trend. After a 2- day adaptation training, the mean escape latency appeared varied across all groups on days 3 and 4, significantly longer in Group C （29, 25）s than in Groups D （17, 14）s and E （16, 13）s, and in Group A （41, 35）s than in Group C. From day 5 onwards, each group showed slower change in mean escape latency. On a daily basis of observation, Groups D and E seemed comparable to each other, while Groups A and B showed basically longer mean escape latency compared with the other 3 groups. Conclusion Manganese staining can be hazardous to learning and memory capacities of rats. In contrast, taurine intervention may be beneficial, suggesting an antagonistic role of taurine against neurotoxicity by Manganese. In addition, age may be an influencing factor for test outcome in the rats.