过氧化物酶体增殖物激活受体δ+294T/C基因多态性与冠心病的关系

Relationship of peroxisome proliferation-activated receptor-delta +294T/C gene polymorphism with coronary heart disease

目的探讨过氧化物酶体增殖物激活受体δ(peroxisome proliferation-activated receptor-delta,PPARδ)+294T/C基因多态性与冠心病的关系。方法运用聚合酶链式反应及限制酶片段长度多态性技术分析无血缘关系汉族人群[包括82例正常对照者(NC),120例冠心病(CHD)患者]的PPARδ+294T/C基因多态性,分析基因型频率、等位基因频率,并对不同基因型患者冠心病的危险性进行评价。结果两组间基因型分布差异有统计学意义(P<0.05);CHD组TC+CC基因型频率及C等位基因频率均明显高于NC组(P<0.05);C等位基因携带(TC+CC)者冠心病危险性高于TT基因型(C等位基因携带者OR:3.16,95%CI:1.39～7.14)。结论PPARδ+294T/C基因多态性与冠心病之间有重要的相关性,C等位基因携带可能是冠心病的的危险因素。

Objective To investigate the relationship between peroxisome proliferation-activated receptor-delta （PPARS） ＋294T/C gene polymorphism and coronary heart disease（CHD）. Methods 202 kinless subjects of the Chinese Han were investigated in this study, including 82 normal controls （NC）, and 120 cases diagnosed as CHD. The PPARδ＋294T/C gene polymorphism was determined by polymerase chain reac-tion and restriction fragment length polymorphisms. The frequencies of PPARδ＋294T/C genotypes and the ＂C＂allele frequency were analyzed, to evaluate the risk for the CHD among variant genotypes. Results The genotype distribution was significantly different between the CHD and the NC （P 〈0.05）.The frequencies of TC＋CC genotype were higher in the CHD group those in the NC, and the CHD risk in the ＂C＂ allele carries was significantly higher than that in the TT homozygote carries （＂C＂allele carries OR：3.16,95 % CI：1.39-7.14）. Conclusion There was evident association between PPARδ＋294T/C gene polymorphism and coronary heart disease, ＂C＂allele carries may be an independent risk factor of the coronary heart disease.