背角无齿蚌(Anodonta woodiana)血细胞的分类

Classification of hemocytes of Anodonta woodiana

用光学显微镜和透射电子显微镜对背角无齿蚌血细胞进行观察.结果发现,体长6～12cm的背角无齿蚌血细胞浓度为(2.42±0.38)×106/mL.根据细胞质内颗粒出现情况,将该蚌血细胞分为两大类,即颗粒细胞和无颗粒细胞.颗粒细胞明显的特征是细胞质中存在许多颗粒,无颗粒细胞的特征是细胞质中不含有或含有少量颗粒.根据颗粒细胞的体积和胞质内颗粒的大小还可以将其分为大颗粒细胞和小颗粒细胞(分别占4.28%和59.28%).根据无颗粒细胞的大小和核质比的不同将其分为透明细胞和淋巴样细胞(分别占27.74%与8.70%).4种类型的血细胞对Wrights染料有不同的亲和性,大颗粒细胞表现为嗜酸性,小颗粒细胞与透明细胞均呈现嗜酸性和嗜碱性两种,淋巴样细胞表现为嗜碱性.

The light microscope and transmission electron microscope were used to obseve hemocytes ofA. woodiana. The results shows that the total hemocyte content ofA. woodiana was about （2.42±0.38）×10^6/ /mL from 6 to 12 centimeters in shell length. Two types of hemocytes were recognized： granular hemocytes and agranular hemocytes,based on either presence or absence of granules in the cytoplasm. Granular hemocytes are distinguished from other hemocytes by a positive feature, the possession of numerous cytoplasmic granules, while agranular hemocytes have few or a few electron-dense cytoplasm granules. In addition, according to the diameter of granular hemocytes and granular size, granular hemocytes were further divided into two categories： large granular hemocytes and small granular hemocytes, about 4.28% and 59.28% in the hemocyte population, respectively. In accordance with the diameter of agranular hemocytes or N/C ratios, agranular hemocytes were further classified into two categories： hyalinocytes and lymphoid hemocytes, about 27.74% and 8.70% in the hemocyte population, respectively. Four types of hemocytes had different tinctorial properties for Wright＇s staining in smear. Large granular hemocytes were acidophils. Both small granular hemocytes and hyalinocytes had two tinctorial properties, acidophils and basophils. Lymphoid hemocytes were only basophils.