高产谷胱甘肽酵母菌株的选育

Breeding of Saccharomyces cerevisiae Strains with High-yield of Glutathione

以BY-14面包酵母(Saccharomyces cerevisiae)为出发菌株,通过紫外线和氮离子注入复合诱变,氯化锌、乙硫氨酸耐性平板筛选,获得一株高产谷胱甘肽的面包酵母优良菌株(S.cerevisiae)BL-23。该菌株经摇瓶发酵谷胱甘肽产量为113.46 mg/L,较出发菌株提高62%,每1 g干细胞含谷胱甘肽20.86 mg,较出发菌株提高56%。传代培养结果表明,该菌株具有稳定的遗传性能。

Saccharomyces cerevisiae BY-14 was used as parent strain and then mutagenized by ultraviolet radiation and ion implantation, and a mutant strain named as S.cerevisiae BL-23 with high-yield of glutathione was obtained through zinc chloride and ethionine tolerance flat screening. The yield of glutathione by BL-23 strain fermentation in shake-flask was 113.46 mg/L, 62 % higher than that by BY-14 strain fermentation, and the content of glutathione in per gram stem cells was 20.86 mg/g, 56 % higher than that by BY-14 strain fermentation. Subculture results showed that such strain had stable heritability.