枸杞多肽对D-半乳糖诱导小鼠的抗衰老作用及其可能机制

Anti-aging effect of polypeptides from Fructus Lycii on D-gal induced aging model mice and the possible mechanism

目的研究枸杞多肽对D-半乳糖(D-gal)衰老模型小鼠的影响及其可能作用机制。方法ICR小鼠60只,随机分为正常对照组、衰老模型组、枸杞多肽200,400,800mg/(kg.d)剂量组和100mg/(kg.d)维生素E(VitE)组。除正常组外均采用D-gal10mg/kg颈背部皮下注射,每日1次,连续注射5周,同时枸杞多肽和VitE组按20ml/(kg.d)灌胃给药。观察各组小鼠的行为学及学习记忆改变,并于5周后检测小鼠血清、心脏、肝脏、脑组织中超氧化物歧化酶(SOD)活性、丙二醛(MDA)含量及端粒酶活性。结果与正常对照组相比,衰老模型组小鼠体重增加明显减少,小鼠跳台错误次数明显增多,小鼠血清、心脏、肝脏和脑SOD和端粒酶活性降低,MDA含量增加(P<0.01)。与模型组相比,枸杞多肽组和VitE组小鼠体重增加升高(P<0.01),小鼠跳台错误次数减少(P<0.05),小鼠血清、心脏、肝脏和脑组织SOD活性升高,MDA含量减少(P<0.05);枸杞多肽200mg/(kg.d)剂量组及VitE组小鼠血清端粒酶活性有升高的趋势,但差异不显著;400和800mg/(kg.d)剂量组小鼠血清和心脏端粒酶活性升高(P<0.01),VitE组小鼠心脏端粒酶活性也明显升高;而各治疗组小鼠肝脏和脑组织端粒酶活性无明显变化。结论枸杞多肽对D-gal诱导衰老模型小鼠有抗衰老作用,其机制可能与提高小鼠血清、心脏、肝脏和脑组织SOD活性,减少MDA含量,以及提高血清和心脏端粒酶活性有关。

Objective To study the anti-aging effect of polypeptides from Fructus Lycii（PFL）on D-galactose（D-gal）induced aging model mice and the possible mechanism.Methods Sixty ICR mice were randomly divided into normal control group,D-gal induced model group,PFL 200,400 and 800 mg/（kg·d）groups and vitamin E（VitE）100 mg/（kg·d）group.D-gal aging mouse model was established by cervicodorsal region subcutaneous injection with D-gal（10 mg/kg）once a day for five successive weeks.In the meantime,drugs were given by intragastric administration respectively in PFL and Vit E treatment groups.The effect of PFL on learning and memory ability of mice was observed.After 5 weeks,the superoxide dismutase（SOD）activity,malondialdehyde（MDA）content and telomerase activity in serum,heart,liver and brain tissues of mice were measured.Results Compared with normal control group,for aging model mice,the weight increasement declined,the number of errors in step-down test increased,the SOD and tolemerase activities in serum heart,liver and brain tissues dropped,and the MDA content was raised,P〈0.01.Compared with model group,for the mice in PFL and VitE treatment groups,the weight increasement rised（P〈0.01）,the error number in step-down test decreased（P〈0.05）,the SOD activity in serum,heart,liver and brain tissues enhanced,and the MDA content reduced（P〈0.01）.The telomerase activity in serum and heart of 400,800 mg/（kg·d）PFL and VitE treatment groups also increased significantly than model group,while that in liver and brain did not change.Conclusion PFL have anti-aging effect on D-gal induced aging mice,and the action mechanism is related to the increasement of SOD activity,the decreasement of MDA content in serum,heart,liver and brain of D-gal aging mice,and the increasement of telomerase activity in serum and heart.