肝细胞癌组织中泛素、P27蛋白表达及两者与临床病理分级的关系

Expression of ubiquitin and P27 protein in hepatocellular carcinoma and its clinicopathologic significance

目的研究人肝细胞癌组织中泛素(ubiquitin)蛋白表达对P27蛋白表达的影响,及两者与肝细胞癌不同病理分级的关系及临床意义。方法采用免疫组织化学法结合蛋白免疫印迹技术,分别检测人肝细胞癌组织不同病理分级中泛素、P27蛋白的表达情况,分析人肝细胞癌组织中泛素、P27蛋白的不同表达情况与临床病理分级之间的相关性。结果肝细胞癌组织中均有泛素、P27蛋白的表达,泛素蛋白定位于细胞核及细胞质,其表达量随分化程度降低反而增高(P<0.05),P27蛋白主要定位于细胞核,其表达量随分化程度降低而降低(P<0.05)。泛素和P27蛋白的表达与病理分级有明显的相关性(P<0.05)。结论泛素蛋白高表达在P27蛋白降解过程中发挥重要作用,并与肝细胞癌的恶性程度、疾病进展有密切的关系。

Objective To study the expression of ubiquitin and P27 protein in hepatocellular carcinoma and its relationship with the different elinicopathologie degrees. Methods Immunohistochemical method and Western blot were used to detect the expression of ubiquitin and P27 protein in hepatocellular carcinoma with different elinicopathologie degrees. Relationship of the expression of ubiquitin and P27 protein in hepatocellular carcinoma with the different clinicopathologie degrees was analyzed. Results The ubiquitin and P27 protein were expressed in hepatocellular carcinoma. Ubiquitin protein was located in nuclei and/or cytoplasm, while P27 protein was mainly located in nuclei. Ubiquitin protein expression was negatively correlated with different clinicopathologic degrees in hepatoeellnlar carcinoma （ P 〈 0.05 ）. P27 protein expression was positively correlated with different clinicopathologic degrees in hepatocellular carcinoma（P 〈 0.05）. Conclusion The higher expression of ubiquitin protein plays an important role in the degradation of P27 protein. The ubiquitin expression and P27 protein expression are closely related to the degree of malignancy and disease progression of human hepatocellular carcinoma.