LC-MS/MS同时测定鸡肉中7种喹诺酮类药物的残留

Simultaneous determination of residues of seven quinolones in chicken muscle by LC-MS/MS

目的建立同时测定鸡肉中7种喹诺酮类(QNs)药物残留的方法。方法鸡肉样品经匀质后用乙腈提取,提取液用正己烷脱脂后过强阳离子固相萃取柱,收集萃取液用氮气吹干,0.2%氨水定容后,采用RP-HPLC/MS/MS法电喷雾电离,多反应监测模式检测。结果在添加浓度20μg·kg-1时,7种QNs的回收率为77.07%～98.71%;RSD均小于7.75%;检测限为0.25～1.07μg·kg-1。结论所建方法灵敏度高、杂质干扰小、特异性强,是鸡肉中QNs残留检测的理想方法。

OBJECTIVE To establish a method for simultaneous determination of seven quinolones （QNs） residues in chicken muscle. METI-IODS Homogenized samples were extracted with acetonitrile. Extracts were first defatted by liquid - liquid partition （LLP） with n -hexane, and then cleaned by sulfonic acid solid phase extraction （SPE） cartridge. Analytes were reconstituted by 0. 2% ammonia after dried under nitrogen. Identification and quantification were achieved by RP - HPLC/MS/MS with electro - spray ionization （ESI） in positive mode and multiple reaction monitoring （MRM） module. RESULTS Recoveries of seven QNs were from 77.07 % to 98.71% at spiked levels of 20 μg·kg^-1. The RSDs of the method were less than 7.75 %. Limits of detection （LOD） for seven QNs were between 0.25 and 1.07 μg·kg^-1. CONCLUSION This method is sensitive, little intefferential and differential for the determination of QNs residues in chicken muscle.