HPLC测定夏枯草中的芦丁和槲皮素

Determination of rutin and quercetin in Prunella vulgaris by HPLC

目的采用HPLC法测定夏枯草中芦丁和槲皮素的含量。方法色谱柱为Alltima C18(250mm×4.6mm,5μm),流动相为甲醇-0.1%冰醋酸,检测波长350nm,流速1.0mL·min-1,柱温30℃。结果芦丁、槲皮素的线性范围分别为0.0149～0.2380mg·mL-1(r=0.9999)、0.0019～0.0306mg·mL-1(r=0.9998),平均回收率分别为97.78%(RSD=0.83%)、101.18%(RSD=0.83%)。安徽、湖北、贵州、江西、广西五产地中芦丁含量分别为0.3527、0、0.2337、2.1164、0.9578mg·g-1生药,槲皮素含量分别为0.0872、0.0473、0.0721、0.5219、0.1755mg·g-1生药。结论所建方法操作简便、准确,可作为夏枯草中芦丁和槲皮素的含量测定方法。

OBJECTIVE To establish an HPLC method for determination of rutin and quercetin in Prunella vulgaris. METHODS The samples were separated on the Alltima C18 （4.6 mm × 250 mm ,5 μm）column which was eluted with methanol and water （0.1% acetic acid） with detective wavelength at 350 nm and flow rate at 1. 0 mL·min^-1, column temperature at 30 ℃. RESULTS The results indicated that the two compounds were basically isolated. The linear ranges of rutin and quercetin were 0. 0149 - 0.2380 mg·mL^-1（r = 0. 9999） ,0. 0019 -0. 0306 mg·mL^-1 （r = 0. 9998 ） respectively. The average recoveries were 97.78% （RSD = 0.83% ）, 101.18% （RSD = 0.83 % ） respectively. The contents of rutin in Prunella vulgaris from Anhui, Hubei, Guizhou, Jiangxi, Guangxi were 0. 3527 ,0,0. 2337,2. 1164, 0. 9578 mg·g^-1 crude drug respectively, and the contents of quercetin were 0. 0872,0. 0473,0. 0721, 0. 5219,0. 1755 mg·g^-1 crude drug respectively. CONCLUSION The method is sensitive, accurate, and can be used to control the quality of Prunella vulgaris. ; Determination.