高产纤维素酶生产菌的筛选及诱变育种

Screening and Mutagenesis of High Cellulase Activity Strain

本研究分离筛选出1株产纤维素酶的木霉(CMC酶活609.51U/g干曲、FPA酶活133.85U/g干曲),以该木霉为出发菌,经紫外线及原生质体紫外线复合诱变育种,获得一株纤维素酶活力较高生产性能稳定的菌株,其CMC酶活达到1332.90U/g干曲,FPA酶活达到301.61U/g干曲,分别是原始菌株的2.19倍,2.25倍。

Use the Tr/choderma （CMC enzyme activity was 609.51U/g dry medium,FPA enzyme activity was 133.85U/g dry medium） as the original strain, a strain with steady high activity cellulase was obtained after UV mutation and protoplast mutagenesis. The CMC enzyme activity was 1332.90U/g dry medium,the FPA enzyme activity was 301.61U/g dry medium which were 2.19 times and 2.25 times higher than the raw strain respectively.