摘要
枯草杆菌Ｂ８２６（ＢａｃｉｌｕｓｓｕｂｔｉｌｉｓＢ８２６）是一株对水稻白叶枯病菌有强烈拮抗作用的细菌．本研究测定了枯草杆菌Ｂ８２６的对数生长曲线并利用化学诱变剂硫酸二乙酯（ＤＥＳ）处理对数生长期的细胞，获得了１６０个存活的菌株，其中６个菌仍保留拮抗活性或拮抗活性有所提高，其余的均丧失拮抗活性．突变菌Ｎｏ．３５能稳定地保持高效的拮抗活性和遗传．
Bacterium strain Bacillus subtilis B826 is one of antagonistic bacteria to rice leaf bacterial blight pathogen, Xanthomonas oryzae pv. oryzae . After treated by chemical mutation regent diethyl sulfate (DES), about 160 survival bacterial clones of B826 were obtained on the KMB medium plate. Most of them lost the antagonistic properties against to X. oryzae pv. oryzae . Six mutants showed significant increasing of antagonistic activity to the pathogen. Among them, clone 35# was studied in detail. After 20 generation, 35# mutant still show very strong antagonistic activity to X. oryzae pv. oryzae .
基金
国家自然科学基金
关键词
枯草杆菌
诱变
拮抗
白叶枯病菌
Bacillus subtilis
induced mutation
antagonistic bacteria
