光合细菌PSB-B4的分离与培养条件优化研究

Isolation and Culture Condition optimization Study of Photosynthetic Bacterium PSB-B4

利用光合细菌处理有机污水是当前污水生物处理中的一种新方法。通过筛选,从污水中分离得到一株生长较快、适应性较强的光合细菌菌株,标记为PSB-B4。通过菌落形态、培养特征和菌体形态学观察,细胞吸收光谱及生理生化特性测定,初步鉴定为类球红细菌(Rhodohactersphaeroides)。对这一光合细菌菌株的培养条件进行优化研究,最终确定菌株PSB-B4的最佳培养基配方及培养条件为:酵母膏2.0 g,乙酸钠4.0 g,氯化铵2.0 g,蛋白胨3.0 g,磷酸氢二钾0.2 g,硫酸镁0.2 g,氯化钠1.25 g,碳酸氢钠1.0 g,蒸馏水1 000 mL,pH=7.0,培养温度28℃,光照强度4 000 Lux,其中酵母膏对其生长影响最大。

The photosynthetic bacteria strain PSB-B4 was isolated from sewage sludge. By enrichment and purification, colonial morphology, culture characteristic, the absorption spectrum of living cells and the determination of physiological and biochemical characteristics, strain PSB-B4 was identified to be Rhodohacter sphaeroides. The optimum composition of culture medium and cultivation parameters of strain PSB-B4 was obtained through optimization research, which is microzyme 2.0 g, sodium acetate 4.0 g, ammonium chloride 2.0 g, peptone 3.0 g, Dipotassium Hydrogen Phosphate 0.2 g, bitter salt 0.2 g, sodium chloride 1.25 g, sodium bicarbonate 1.0 g, distilled water 1 000 mL, pHT. 0, temperature 30 ℃, Light 4 000 Lux. In which microzyme has greatest impact on the growth of strain PSB-B4.