人源性乳腺移植模型:有效提高人源性乳腺癌细胞株在小鼠原位成瘤率的途径

Human breast tissue implantation favors breast cancer xenograft formation in nude mice

目的:探索解决部分人乳腺癌细胞株在小鼠模型中成瘤率过低问题,以稳定提高乳腺癌小鼠模型的成瘤效率。方法:在雌性重症联合免疫缺陷症(severe combined immunodeficiency,SCID)小鼠的一侧胁腹皮下移植人源性正常乳腺组织,向移植组织内注射绿色荧光蛋白(GFP)标记的人乳腺癌细胞株MCF-7;同时以传统的小鼠乳腺脂肪垫(MFP)注射模型作为对照,即在雌性SCID小鼠一侧腹股沟MFP内注射同等数量GFP标记的MCF-7。从活体动物荧光成像仪和病理检测两个水平评估移植模型和MFP注射模型的原位成瘤率。结果:人源性乳腺移植小鼠模型的原位成瘤率在大体荧光观察下为95%(19/20例),在病理检测中为100%(20/20例);MFP注射模型的原位成瘤率在大体荧光观察下为0(0/20例),在病理检测中为15%(3/20例)。人源性乳腺移植模型在大体(P<0.05)和镜下(P<0.05)两个水平的成瘤率都远高于传统的MFP注射模型。结论:人源性乳腺移植模型可显著提高人乳腺癌细胞株在小鼠模型上的原位成瘤率。

Objective To improve the efficiency and stability of breast cancer xenografts formation in nude mice. Methods Normal human-derived breast tissue was implanted into the flanks of female severe combined immunodeficiency （SCID） mice subcutaneously, then followed by GFP-labeled human breast cancer cell line MCF-7 （experimental group） . Meanwhile, directly inoculation of MCF-7 ceils into the mammalian fatty pad （MFP） were served as the control group. The rate of the xenograft formation was evaluated by both the Fluorescent image system and pathologic examination. Results The rate of the xenograft formation was 95% or 19/20 macroscopically in experimental group as compared with 0 or 0/20 in the control group （P〈0.05）. Microscopically, the rate of xenografl formation was 100% （20/20） in the experimental group and 15% （3/20） in the control group （P〈0.05）. Conclusions The human breast tissue derived graft can significantly improve the rate of human breast cancer xenograft formation in nude mice.