摘要
The purpose of this study was to investigate the antibacterial activity of extracts from Zostera marina against the pathogens of Apostichopus japonicus skin ulceration disease.When 95% ethanol (v/v) solvent was used to extract Zostera marina at 50℃,aqueous extract (ZA) showed obvious bacteriostatic effects on the tested bacterial strains (inhibition halo diameters between 8.23 mm and 13.62 mm),whereas the ethyl acetate extract (ZE) was almost inactive.The minimal inhibitory concentration (MIC) of ZA against four pathogens were homogeneous at 12.8 g L-1.ZA components were analyzed by thin layer chromatography (TLC) assay and six fractions were obtained.In another study,the six fractions showed inhibitory effects against the tested bacteria while their functions seemed to counteract the ZA activity.
The purpose of this study was to investigate the antibacterial activity of extracts from Zostera marina against the pathogens of Apostichopusjaponicus skin ulceration disease. When 95% ethanol （v/v） solvent was used to extract Zostera marina at 50℃, aqueous extract （ZA） showed obvious bacteriostatic effects on the tested bacterial strains （inhibition halo diameters between 8.23 mm and 13.62mm）, whereas the ethyl acetate extract （ZE） was almost inactive. The minimal inhibitory concentration （MIC） of ZA against four pathogens were homogeneous at 12.8 g L^-1. ZA components were analyzed by thin layer chromatography （TLC） assay and six fractions were obtained. In another study, the six fractions showed inhibitory effects against the tested bacteria while their functions seemed to counteract the ZA activity.
基金
supported by the National Key Technology Research and Development Program (2007BAD 62B04)
