IL-17F在银屑病皮损中的表达及意义

The Expression and Significant of IL-17F in Psoriatic Lesions

目的探讨IL-17F在银屑病皮损中的表达及意义。方法收集寻常性银屑病患者皮损及正常皮肤作为对照,荧光定量PCR法检测两种组织中IL-17F和IL-8表达的差异;IL-17F诱导人角质形成细胞系HaCaT24h后,荧光定量PCR法检测IL-8的表达变化。结果银屑病患者皮损IL-17F表达明显高于正常组,差异有统计学意义(P均<0.01);银屑病患者皮损同时伴有IL-8的高表达;IL-17F可诱导HaCaT细胞系IL-8的表达增高。结论IL-17F在银屑病皮损中表达上调,IL-17F可诱导HaCaT产生IL-8,这可能是IL-17F参与银屑病发生和发展的机制之一。

Objective To investigate the change of IL-17F in psoriatic lesions. Methods The lesions of vulgaris psoriatic patient and normal control were collected. Fluorescent quantitation PCR was adopted to detect the level of IL-17F in two groups. Meanwhile the expression of IL-8 was detected by same methods, Human keratinocyte cell line, HaCaT cells were induced by IL-17F for 24h, and the expression change of IL-8 were detected. Result The level of IL-17F,IL-8 were higher in psoriatic lesions than in normal tissue. The difference was statistical significance. After treatment with IL-17F, the expression of IL-8 increased hugely. Conclusion The expression level of IL-17F and IL-8 in psoriatic lesions were increased comparing with normal tissue. IL-17F could induce the expression of IL-8 in HaCaT, which may play important role in the occurrence and develop of psoriasis.