IKB激酶在系统性红斑狼疮患者中表达及与干扰素-α产生的关系

The expression of IKB kinase in the peripheral blood and its correlation with the production of interferon-α in systemic lupus erythematosus patients

目的研究系统性红斑狼疮（SEE）患者外周血白细胞中IKB激酶（IKK-α）、干扰素（IFN）-αmRNA的表达，并检测血浆中IFN-α的水平，以探讨SLE患者中IKK—α在IFN—α产生中的作用。方法SYB Rgreen dye I实时定量聚合酶链反应（PCR）方法检测外周血白细胞IKK-α和IFN-α的表达；酶联免疫吸附试验（ELISA）法检测血清IFN吨的水平。结果①SLE患者外周血IKK-αmRNA表达高于对照组（P〈0．05）；在活动组SLE患者中IKK—αmRNA的表达高于非活动组SLE患者（P〈0．01）。②SLE患者IFN-αmRNA的表达低于对照组（P〈0．01），IFN-αmRNA的表达在非活动组SLE患者中低于活动组SLE患者（P〈0．01）。③SLE患者血清中IFN-α的水平高于对照组（P〈0．01），其中，活动组SLE患者血浆IFN-α水平显著高于非活动组患者（P〈0．05）；SLE患者血浆中IFN-α浓度与抗双链DNA（dsDNA）抗体呈正相关（P=001），与补体C3水平呈负相关（P=0．005）。④SLE患者IKK-αmRNA的表达与血浆中IFN-α的水平呈正相关（P=0．001）.结论SLE患者IKK-αmRNA的表达明显增高，且与血浆中IFN-α的水平呈正相关；而血浆中IFN—α的水平与SLE的发病及病情活动相关，提示IKK-α可能在SLE的发病中发挥重要的作用。

Objective To investigate the mRNA expression of IKB kinase （IKK-α） and interferon-ix （IFN-α） in the peripheral blood leukocytes of patients with systemic lupus erythematosus （SLE）, and to explore the role of IKK-α in the production of IFN-α in SLE patients. Methods SYBR green dye I based real-time quantitative PCR was used to detect the mRNA expression levels of IKK-α and IFN-α in the peripheral blood leucocytes of SLE patients and healthy controls. Serum levels of IFN-α were measured with ELISA method. Results IKK-αmRNA expression levels in SLE patients were significantly higher than those of normal controls （P〈0.05）. IKK-α mRNA expression levels in SLE patients with active disease were significantly higher than patients with stable disease （P〈0.01）. IFN-α mRNA expression level in SLE patients was significantly lower than that of the normal controls （P〈0.01）. IFN-αmRNA expression levels in SLE patients with active disease were significantly higher than patients with stable disease （P〈0.01）. Serum levels of IFN-α in SLE patients with active disease was significantly higher than that of the normal controls and patients with stable disease （P〈0.05）. The anti-dsDNA antibody correlated positively, and complement C3 correlated negatively with serum concentration of IFN-α. IKK-αmRNA expression levels in SLE patients correlated positively with serum concentration of IFN-α. Conclusion IKK-α correlates positively with serum concentration of IFN-α. The IFN-α level is significantly correlated with disease activity. This suggests that IKK-α may play an important role in the pathogenesis of SLE.