摘要
目的:探讨血管生成素-2(Ang-2)及其受体具有免疫球蛋白和表皮生长因子同源性结构域的酪氨酸蛋白激酶-2(Tie-2)在大鼠急性肺损伤(ALI)时的表达及意义。方法:42只Wistar大鼠随机分为对照组12只,采用假手术处理;CLP组30只,采用盲肠结扎穿孔术(CLP)制作ALI模型,于假手术及CLP术后12h每组各处死6只大鼠,进行动脉血气分析,取左肺计算肺系数,采用免疫组织化学法检测Tie-2及Ang-2在鼠肺组织中的表达,酶联免疫吸附试验检测血清Ang-2的水平,观察36h内2组鼠死亡率。结果:光镜下对照组鼠肺组织结构清楚;CLP组肺泡间隔增厚,毛细血管充血水肿,有中性粒细胞及巨噬细胞浸润。对照组在支气管上皮细胞、平滑肌细胞及内皮细胞表达Tie-2;CLP组Tie-2表达与对照组相似外,中性粒细胞及巨噬细胞有强阳性表达,在Tie-2阳性的炎症细胞附壁区域,内皮细胞Tie-2表达亦增强。对照组Ang-2主要表达于平滑肌细胞、支气管上皮细胞及内皮细胞,CLP组Ang-2在炎症渗出区的巨噬细胞表达增强,其它与对照组相似。CLP组血清Ang-2水平(8.14±1.74)μg/L明显高于对照组(4.63±0.49)μg/L(P<0.01),且CLP组36h内死亡鼠Ang-2血清水平(8.95±1.61)μg/L显著高于存活鼠(6.80±0.96)μg/L(P<0.01);CLP组动脉血氧分压低于对照组(P<0.01),肺系数则高于对照组(P<0.01)。结论:Ang-2与Tie-2可能参与ALI的发病过程,Ang-2血清水平增高与预后有关。
AIM： To explore the expressions and significance of angiopoietin -2 （Ang -2） and tyrosine kinase with immunoglobulin and epidermal growth factor homology domains -2 （Tie -2 ） receptors in a rat model of acute lung injury （ALI）. METHODS： Wistar rats （ n = 42 ） were divided into control group （ n = 12 ） and cecal ligation and puncture （CLP） group （n=30）. Control group underwent sham operation, and CLP group underwent cecal ligation and puncture to make the model of ALl. 12 h after sham operation or CLP, 6 rats in each group were killed, and arterial blood gas analysis and lung coefficient were tested. The expressions of Ang - 2 and Tie - 2 receptors in lung tissue were observed by immunohistochemical method. Blood samples of the rest rats were collected from vena caudalis, and Ang - 2 levels were measured by enzyme linked immunosorbent assay （ELISA）. The mortality rate in each group within 36 h was compared. The lung architecture was observed under microscope. RESULTS ： The lung architecture in control group was clear and intact. Alveolar septum was thicker, blood capillary was congested, and neutrophils and macrophages were infiltrated in the lung tissue in CLP group. Tie -2 receptors were expressed in bronchial epithelial cells, smooth muscle cells and endothelial cells in control group. Besides the similar expression as control group, high expression of Tie - 2 on neutrophils and macrophages in CLP group was observed. In the adhesion location of Tie - 2 receptors positive inflammatory ceils, there was stronger staining in endothelial cells. Ang -2 was expressed in smooth muscle cells, bronchial epithelial ceils and endothelial cells in control group. The Ang - 2 level in CLP group were higher than that in control group [ （ 8. 14± 1.74 ） μg/L vs （4. 63±0. 49）μg/L, P 〈0. 01 ], and the Ang -2 level of dead rats was higher than that of survival rats within 36 h in CLP group [ （8.95 ±1.61 ） μg/L vs （6. 80 ±0. 96） μg/L, P 〈0.01 ]. Oxygen partial pressure in control group was lower （ P 〈 0. 01 ） and lung coefficient was higher （ P 〈 0. 01 ） than that in CLP group. CONCLUSION ： Ang - 2 and Tie - 2 receptors may participate in the pathophysiology of ALl, and Ang - 2 level is correlated with mortality.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
2010年第2期314-317,共4页
Chinese Journal of Pathophysiology
基金
山东省科技厅自然科学基金资助项目(No.Y2008C163)
